Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde,Microchimica Acta

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Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-11-22 , DOI: 10.1007/s00604-019-3944-1
Tapas Kumar Mandal ₁ , Nargish Parvin ₂ , Kanchan Mishra ₁ , Sonaimuthu Mohandoss ₁ , Yong Rok Lee ₁

Affiliation

A modified method is described for the preparation of amino-functionalized covalent organic framework nanosheets (COF-NSs). These consist of hexagonal layered sheets and were prepared from commercially available starting materials (p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde). The interlayer stacking interactions between the ultra-thin COF-NSs became weak because the π stacking is destroyed by sonication. This result in the exfoliation of COF-NSs. As an application, the COF-NSs used for sensitive and selective fluorometric determination of DNA. To reach this goal, H1 and H2 hairpin-like DNA probes were chosen; H1 used Texas Red-labeled dye as a fluorescent probe. The addition of the COF-NSs, the hairpin probes was adsorbed onto the porous surface of the COFNSs. The π stacking and hydrogen-bond interactions between COFNSs and nucleic acid quench the fluorescence of the Texas red-labeled probe. The target DNA enables the recovery of the quenched fluorescence of the Texas red-labelled probe by triggering an inter-chain hybridization within hairpin probes. This results in a weaker interaction of double-stranded DNA (dsDNA) with the COFNSs. Consequently, the dsDNA detaches from the COFNSs, thereby recovering the dye’s fluorescence (excitation/emission maxima at 590/612 nm) with increasing target DNA concentration. The findings were applied to design a method for the determination of DNA that has a 2 pM detection limit. This is significantly lower than the limit of detection reported previously for 2D nanomaterial-based fluorometric DNA assays. Graphical abstract Schematic representation of 2D-covalent organic framework nanosheets (COF-NSs) probe act as a quencher allowing the highly sensitive and selective fluorescence turn-on detection for biomolecules. Here the H1 H2 are hairpin DNAs. H1 is associated with the fluorescent tag (red circle), while the "fluorescence off" state it denoted as a black circle. Schematic representation of 2D-covalent organic framework nanosheets (COF-NSs) probe act as a quencher allowing the highly sensitive and selective fluorescence turn-on detection for biomolecules. Here the H1 H2 are hairpin DNAs. H1 is associated with the fluorescent tag (red circle), while the "fluorescence off" state it denoted as a black circle.

中文翻译：

使用由对苯二胺和苯-1,3,5-三甲醛制备的共价有机骨架的层状六边形纳米片对 DNA 进行灵敏和选择性的荧光测定

描述了一种用于制备氨基官能化共价有机骨架纳米片 (COF-NSs) 的改进方法。它们由六边形层状片材组成，由市售原材料（对苯二胺和苯-1,3,5-三甲醛）制备。由于 π 堆积被超声破坏，超薄 COF-NS 之间的层间堆积相互作用变弱。这导致 COF-NSs 的剥离。作为一种应用，COF-NSs 用于 DNA 的灵敏和选择性荧光测定。为了达到这个目标，我们选择了 H1 和 H2 发夹样 DNA 探针；H1 使用德克萨斯红标记的染料作为荧光探针。添加 COF-NSs，发夹探针被吸附到 COFNSs 的多孔表面上。COFNS 与核酸之间的 π 堆积和氢键相互作用淬灭了德克萨斯红标记探针的荧光。目标 DNA 通过触发发夹探针内的链间杂交，能够恢复德克萨斯红色标记探针的淬灭荧光。这导致双链 DNA (dsDNA) 与 COFNS 的相互作用较弱。因此，dsDNA 与 COFNS 分离，从而随着目标 DNA 浓度的增加而恢复染料的荧光（激发/发射最大值在 590/612 nm）。这些发现被用于设计一种检测 DNA 检测限为 2 pM 的方法。这明显低于之前报道的基于 2D 纳米材料的荧光 DNA 检测的检测限。图形摘要二维共价有机骨架纳米片 (COF-NSs) 探针的示意图作为淬灭剂，允许对生物分子进行高灵敏度和选择性的荧光开启检测。这里的 H1 H2 是发夹 DNA。H1 与荧光标记（红色圆圈）相关联，而“荧光关闭”状态则表示为黑色圆圈。二维共价有机骨架纳米片 (COF-NSs) 探针的示意图作为淬灭剂，允许对生物分子进行高灵敏度和选择性的荧光开启检测。这里的 H1 H2 是发夹 DNA。H1 与荧光标记（红色圆圈）相关联，而“荧光关闭”状态则表示为黑色圆圈。

更新日期：2019-11-22

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