RNase III和RNase G的协同作用控制烯醇酶的表达，以响应大肠杆菌中的氧气供应。,Scientific Reports

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RNase III和RNase G的协同作用控制烯醇酶的表达，以响应大肠杆菌中的氧气供应。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-11-21 , DOI: 10.1038/s41598-019-53883-y
Minho Lee ₁ , Minju Joo ₁ , Minji Sim ₁ , Se-Hoon Sim ₁ , Hyun-Lee Kim ₁ , Jaejin Lee ₁ , Minkyung Ryu ₁ , Ji-Hyun Yeom ₁ , Yoonsoo Hahn ₁ , Nam-Chul Ha ₂ , Jang-Cheon Cho ₃ , Kangseok Lee ₁

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已知在对环境变化的生理反应期间，通过内切核糖核酸酶快速调节RNA功能是一种有效的细菌生化适应方法。我们报告了由两个内切核糖核酸酶，RNase G和RNase III调节烯醇酶（eno）表达的分子机制，其表达水平受大肠杆菌中的氧利用率调节。转录的eno-cat融合构建体的分析强烈建议在响应RNase III和RNase G细胞浓度的eno 5'非翻译区中存在顺式作用元件。体内eno mRNA的引物延伸和S1核酸酶作图分析鉴定了三种eno mRNA转录物，这些转录物以依赖于RNase III表达的方式产生，其中之一在rng缺失的细胞中积累。而且，我们的数据表明，RNase III介导的主要eno mRNA转录产物的切割增强了Eno蛋白的产生，这一过程涉及推定的顺反义RNA。我们发现降低的RNase G蛋白丰度与厌氧生长的大肠杆菌中增强的RNase III表达相吻合，从而导致eno表达增强。因此，eno表达的这种转录后上调有助于大肠杆菌细胞将其生理反应调节至缺氧的代谢模式。我们的研究结果揭示了协调的内切核糖核酸酶活性的分子网络，该分子网络在转录后调节糖酵解中的关键酶Eno的表达。我们发现降低的RNase G蛋白丰度与厌氧生长的大肠杆菌中增强的RNase III表达相吻合，从而导致eno表达增强。因此，eno表达的这种转录后上调有助于大肠杆菌细胞将其生理反应调节至缺氧的代谢模式。我们的研究结果揭示了协调的内切核糖核酸酶活性的分子网络，该分子网络在转录后调节糖酵解中的关键酶Eno的表达。我们发现降低的RNase G蛋白丰度与厌氧生长的大肠杆菌中增强的RNase III表达相吻合，从而导致eno表达增强。因此，eno表达的这种转录后上调有助于大肠杆菌细胞将其生理反应调节至缺氧的代谢模式。我们的研究结果揭示了协调的内切核糖核酸酶活性的分子网络，该分子网络在转录后调节糖酵解中的关键酶Eno的表达。

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The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli.

Rapid modulation of RNA function by endoribonucleases during physiological responses to environmental changes is known to be an effective bacterial biochemical adaptation. We report a molecular mechanism underlying the regulation of enolase (eno) expression by two endoribonucleases, RNase G and RNase III, the expression levels of which are modulated by oxygen availability in Escherichia coli. Analyses of transcriptional eno-cat fusion constructs strongly suggested the existence of cis-acting elements in the eno 5' untranslated region that respond to RNase III and RNase G cellular concentrations. Primer extension and S1 nuclease mapping analyses of eno mRNA in vivo identified three eno mRNA transcripts that are generated in a manner dependent on RNase III expression, one of which was found to accumulate in rng-deleted cells. Moreover, our data suggested that RNase III-mediated cleavage of primary eno mRNA transcripts enhanced Eno protein production, a process that involved putative cis-antisense RNA. We found that decreased RNase G protein abundance coincided with enhanced RNase III expression in E. coli grown anaerobically, leading to enhanced eno expression. Thereby, this posttranscriptional up-regulation of eno expression helps E. coli cells adjust their physiological reactions to oxygen-deficient metabolic modes. Our results revealed a molecular network of coordinated endoribonuclease activity that post-transcriptionally modulates the expression of Eno, a key enzyme in glycolysis.

更新日期：2019-11-21

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