Modulating the Biofunctionality of Metal-Organic-Framework-Encapsulated Enzymes through Controllable Embedding Patterns.,Angewandte Chemie International Edition

当前位置： X-MOL 学术 › Angew. Chem. Int. Ed. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Modulating the Biofunctionality of Metal-Organic-Framework-Encapsulated Enzymes through Controllable Embedding Patterns.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2019-11-21 , DOI: 10.1002/anie.201913231
Guosheng Chen ₁ , Xiaoxue Kou ₁ , Siming Huang ₂ , Linjing Tong ₁ , Yujian Shen ₁ , Wangshu Zhu ₂ , Fang Zhu ₁ , Gangfeng Ouyang _{1,

3}

Affiliation

Embedding an enzyme within a MOF as exoskeleton (enzyme@MOF) offers new opportunities to improve the inherent fragile nature of the enzyme, but also to impart novel biofunctionality to the MOF. Despite the remarkable stability achieved for MOF-embedded enzymes, embedding patterns and conversion of the enzymatic biofunctionality after entrapment by a MOF have only received limited attention. Herein, we reveal how embedding patterns affect the bioactivity of an enzyme encapsulated in ZIF-8. The enzyme@MOF can maintain high activity when the encapsulation process is driven by rapid enzyme-triggered nucleation of ZIF-8. When the encapsulation is driven by slow coprecipitation and the enzymes are not involved in the nucleation of ZIF-8, enzyme@MOF tends to be inactive owing to unfolding and competing coordination caused by the ligand, 2-methyl imidazole. These two embedding patterns can easily be controlled by chemical modification of the amino acids of the enzymes, modulating their biofunctionality.

中文翻译：

通过可控的嵌入模式调节金属有机框架封装的酶的生物功能。

将酶嵌入到MOF中作为外骨骼（enzyme @ MOF）提供了新的机会来改善酶的固有易碎性，同时也为MOF赋予了新的生物功能。尽管嵌入MOF的酶具有显着的稳定性，但是MOF诱捕后的嵌入模式和酶生物功能的转化仅受到了有限的关注。在这里，我们揭示了嵌入模式如何影响ZIF-8中封装的酶的生物活性。当酶促ZIF-8快速成核驱动包封过程时，酶@MOF可以保持高活性。当封装是由缓慢的共沉淀驱动的，并且酶不参与ZIF-8的成核时，由于配体的展开和竞争性配位，酶@MOF趋于失活，2-甲基咪唑。可以通过化学修饰酶的氨基酸来调节这两种嵌入方式，从而调节其生物功能。

更新日期：2020-01-04

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南