<br>绘制光遗传学驱动的单血管功能磁共振成像与大鼠海马中并发神经元钙记录。,Nature Communications

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绘制光遗传学驱动的单血管功能磁共振成像与大鼠海马中并发神经元钙记录。
Nature Communications ( IF 14.7 ) Pub Date : 2019-11-20 , DOI: 10.1038/s41467-019-12850-x
Xuming Chen _{1,

2,

3} , Filip Sobczak _{1,

4} , Yi Chen _{1,

4} , Yuanyuan Jiang _{1,

5} , Chunqi Qian ₆ , Zuneng Lu ₃ , Cenk Ayata _{7,

8} , Nikos K Logothetis _{9,

10} , Xin Yu _{1,

5}

Affiliation

Research Group of Translational Neuroimaging and Neural Control, High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, 72076, Tuebingen, Germany.
University of Tuebingen, 72074, Tuebingen, Germany.
Department of Neurology, Wuhan University, Renmin Hospital, Wuhan, 430060, China.
Graduate Training Centre of Neuroscience, International Max Planck Research School, University of Tuebingen, 72074, Tuebingen, Germany.
Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, Charlestown, 02129, MA, USA.
Department of Radiology, Michigan State University, East Lansing, 48824, MI, USA.
Neurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, 02129, MA, USA.
Stroke Service and Neuroscience Intensive Care Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, 02129, Boston, USA.
Department of Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Tuebingen, 72076, Germany.
Department of Imaging Science and Biomedical Engineering, University of Manchester, Manchester, M13 9PT, UK.

广泛的体内成像研究调查了海马神经网络功能，鉴于其光学可及性，主要集中在背侧 CA1 区域。多模态功能磁共振成像与同时海马电生理记录揭示了广泛的皮质相关模式，但由于功能磁共振成像分辨率有限，仍然缺乏详细的空间海马功能图。特别是，与特定神经活动相关的血流动力学反应在海马脉管系统的单血管水平上尚不清楚，这阻碍了动物模型中海马功能障碍的破译以及人类功能磁共振成像关键神经血管耦合（NVC）模式的转化。我们同时从单个小静脉和小动脉获取了光遗传学驱动的神经元 Ca2+ 信号，以及单血管血氧水平依赖性 (BOLD) 和脑血容量 (CBV)-fMRI。海马血流动力学反应的独特时空模式与光遗传学诱发和传播的抑郁样钙事件相关。钙事件相关的单血管血流动力学模型显示，在扩散抑郁样（SDL）事件时，NVC 效率显着降低，从而提供了各种海马状态下 NVC 功能的直接测量。

"点击查看英文标题和摘要"

Mapping optogenetically-driven single-vessel fMRI with concurrent neuronal calcium recordings in the rat hippocampus.

Extensive in vivo imaging studies investigate the hippocampal neural network function, mainly focusing on the dorsal CA1 region given its optical accessibility. Multi-modality fMRI with simultaneous hippocampal electrophysiological recording reveal broad cortical correlation patterns, but the detailed spatial hippocampal functional map remains lacking given the limited fMRI resolution. In particular, hemodynamic responses linked to specific neural activity are unclear at the single-vessel level across hippocampal vasculature, which hinders the deciphering of the hippocampal malfunction in animal models and the translation to critical neurovascular coupling (NVC) patterns for human fMRI. We simultaneously acquired optogenetically-driven neuronal Ca2+ signals with single-vessel blood-oxygen-level-dependent (BOLD) and cerebral-blood-volume (CBV)-fMRI from individual venules and arterioles. Distinct spatiotemporal patterns of hippocampal hemodynamic responses were correlated to optogenetically evoked and spreading depression-like calcium events. The calcium event-related single-vessel hemodynamic modeling revealed significantly reduced NVC efficiency upon spreading depression-like (SDL) events, providing a direct measure of the NVC function at various hippocampal states.

更新日期：2019-11-20

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