肉桂醛醇脱氢酶缺失水稻的木质纤维素化学结构和分子组装发生变化。,Scientific Reports

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肉桂醛醇脱氢酶缺失水稻的木质纤维素化学结构和分子组装发生变化。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-11-20 , DOI: 10.1038/s41598-019-53156-8
Andri Fadillah Martin _{1,

2} , Yuki Tobimatsu ₁ , Ryosuke Kusumi ₃ , Naoyuki Matsumoto ₁ , Takuji Miyamoto ₁ , Pui Ying Lam ₁ , Masaomi Yamamura ₁ , Taichi Koshiba ₁ , Masahiro Sakamoto ₃ , Toshiaki Umezawa _{1,

4}

Affiliation

木质素是沉积在植物细胞壁中的一种复杂的苯基丙烷类聚合物。长期以来，木质素一直是多糖类生物质利用的重要限制因素。为了减轻木质素相关生物量的顽固性，已经产生并表征了许多产生具有降低的木质素含量的木质纤维素和/或具有改变的化学结构的木质素的突变体和转基因植物。然而，尚未完全理解改变的木质素化学如何影响木质纤维素的超分子结构，并因此影响其利用性质。在本文中，我们对由缺少肉桂酸乙醇脱氢酶（CAD）的水稻cad2突变体产生的木质纤维素进行了全面的化学和超分子结构分析，该突变体编码木质素生物合成中的关键酶。通过使用溶液状态二维NMR方法和互补的化学方法，我们阐明了富含cad2突变体产生的不寻常的羟基肉桂醛衍生的亚结构的改变后的木质素的结构细节。同时，通过固态13 C MAS NMR，核磁弛豫，X射线衍射和Simon染色分析研究了多糖的组装和木质纤维素的分子迁移率。在cad2水稻突变体提高生物质糖化效率的背景下，讨论了与CAD相关的木质素修饰（就总含量和化学结构而言）与木质纤维素超分子结构变化之间的可能联系。我们阐明了由cad2突变体产生的富含异常的羟基肉桂醛衍生的亚结构的木质素改变后的结构细节。同时，通过固态13 C MAS NMR，核磁弛豫，X射线衍射和Simon染色分析研究了多糖的组装和木质纤维素的分子迁移率。在cad2水稻突变体提高生物质糖化效率的背景下，讨论了与CAD相关的木质素修饰（就总含量和化学结构而言）与木质纤维素超分子结构变化之间的可能联系。我们阐明了由cad2突变体产生的富含异常的羟基肉桂醛衍生的亚结构的木质素改变后的结构细节。同时，通过固态13 C MAS NMR，核磁弛豫，X射线衍射和Simon染色分析研究了多糖的组装和木质纤维素的分子迁移率。在cad2水稻突变体提高生物质糖化效率的背景下，讨论了CAD相关的木质素修饰（就总含量和化学结构而言）与木质纤维素超分子结构变化之间的可能联系。核磁弛豫，X射线衍射和Simon染色分析。在cad2水稻突变体提高生物质糖化效率的背景下，讨论了与CAD相关的木质素修饰（就总含量和化学结构而言）与木质纤维素超分子结构变化之间的可能联系。核磁弛豫，X射线衍射和Simon染色分析。在cad2水稻突变体提高生物质糖化效率的背景下，讨论了与CAD相关的木质素修饰（就总含量和化学结构而言）与木质纤维素超分子结构变化之间的可能联系。

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Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice.

Lignin is a complex phenylpropanoid polymer deposited in plant cell walls. Lignin has long been recognized as an important limiting factor for the polysaccharide-oriented biomass utilizations. To mitigate lignin-associated biomass recalcitrance, numerous mutants and transgenic plants that produce lignocellulose with reduced lignin contents and/or lignins with altered chemical structures have been produced and characterised. However, it is not fully understood how altered lignin chemistry affects the supramolecular structure of lignocellulose, and consequently, its utilization properties. Herein, we conducted comprehensive chemical and supramolecular structural analyses of lignocellulose produced by a rice cad2 mutant deficient in CINNAMYL ALCOHOL DEHYDROGENASE (CAD), which encodes a key enzyme in lignin biosynthesis. By using a solution-state two-dimensional NMR approach and complementary chemical methods, we elucidated the structural details of the altered lignins enriched with unusual hydroxycinnamaldehyde-derived substructures produced by the cad2 mutant. In parallel, polysaccharide assembly and the molecular mobility of lignocellulose were investigated by solid-state 13C MAS NMR, nuclear magnetic relaxation, X-ray diffraction, and Simon's staining analyses. Possible links between CAD-associated lignin modifications (in terms of total content and chemical structures) and changes to the lignocellulose supramolecular structure are discussed in the context of the improved biomass saccharification efficiency of the cad2 rice mutant.

更新日期：2019-11-20

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