使用负载有 N 掺杂碳量子点的氨基修饰还原氧化石墨烯对神经元特异性烯醇化酶进行电化学发光免疫分析,Microchimica Acta

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使用负载有 N 掺杂碳量子点的氨基修饰还原氧化石墨烯对神经元特异性烯醇化酶进行电化学发光免疫分析
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-11-20 , DOI: 10.1007/s00604-019-3986-4
Xiangfei Zheng ₁ , Guichun Mo ₁ , Yongyu He ₁ , Dongmiao Qin ₁ , Xiaohua Jiang ₁ , Weiming Mo ₁ , Biyang Deng ₁

Affiliation

提出了一种基于超灵敏电化学发光的夹心免疫测定法，用于测定神经元特异性烯醇化酶。该方法使用银-半胱氨酸纳米线作为捕获探针，由氨基修饰的还原氧化石墨烯和掺氮碳量子点组成的复合物作为信号探针。它是通过氨基修饰的还原氧化石墨烯与氮掺杂碳量子点的羧基共价偶联合成的。纳米线具有大的比表面积和丰富的官能团，有助于固定一抗 (Ab1)。氨基修饰的还原氧化石墨烯被用作负载大量量子点和二抗（Ab2）的载体。这增加了量子点的电化学发光强度。对神经元特异性烯醇化酶的反应在 0.55 fg·mL-1 至 5.5 ng·mL-1 浓度范围内呈线性。它的检测限为 0.18 fg·mL-1（S/N = 3）。相对标准偏差（对于 n = 6）小于 2.9%。该测定具有高度灵敏性、可重复性、选择性和稳定性。图形摘要描述了一种新型电化学发光免疫传感器，它使用氨基修饰的还原氧化石墨烯 (amino-rGO)、氮掺杂碳量子点 (N-CQD) 和银半胱氨酸纳米线 (SCNW)。应用于神经元特异性烯醇化酶（NSE）的测定。牛血清白蛋白：BSA；1-乙基-3-（3-二甲氨基丙基）碳二亚胺：（EDC；，N-羟基琥珀酰亚胺：NHS。描述了一种使用氨基修饰的还原氧化石墨烯（氨基-rGO）的新型电化学发光免疫传感器，氮掺杂碳量子点（N-CQDs）和银半胱氨酸纳米线（SCNWs）。应用于神经元特异性烯醇化酶（NSE）的测定。牛血清白蛋白：BSA；1-乙基-3-（3-二甲氨基丙基）碳二亚胺：（EDC；，N-羟基琥珀酰亚胺：NHS。

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Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots

An ultrasensitive electrochemiluminescence based sandwich immunoassay is presented for determination of neuron specific enolase. The method uses silver-cysteine nanowires as the capture probe and a composite made of amino-modified reduced graphene oxide and nitrogen-doped carbon quantum dots as the signal probe. It was synthesized by covalent coupling of amino-modified reduced graphene oxide to the carboxy groups of nitrogen-doped carbon quantum dots. The nanowires possess a large specific surface and abundant functional groups which facilitate immobilizing the primary antibody (Ab1). The amino-modified reduced graphene oxide is employed as a carrier for loading a large number of the quantum dots and secondary antibody (Ab2). This increases the electrochemiluminescence intensity of quantum dots. Response to neuron specific enolase is linear in the 0.55 fg·mL−1 to 5.5 ng·mL−1 concentration range. It has a detection limit of 0.18 fg·mL−1 (at S/N = 3). The relative standard deviation (for n = 6) is less than 2.9%. The assay is highly sensitive, reproducible, selective and stable. Graphical abstract A novel electrochemiluminescence immunosensor is described that uses amino-modified reduced graphene oxide (amino-rGO), nitrogen-doped carbon quantum dots (N-CQDs) and silver-cysteine nanowires (SCNWs). It was applied to the determination of neuron specific enolase (NSE). Bovine serum albumin: BSA;1-ethyl-3-(3-dimethylaminopropyl)carbodiimide: (EDC;, N-hydroxysuccinimide: NHS. A novel electrochemiluminescence immunosensor is described that uses amino-modified reduced graphene oxide (amino-rGO), nitrogen-doped carbon quantum dots (N-CQDs) and silver-cysteine nanowires (SCNWs). It was applied to the determination of neuron specific enolase (NSE). Bovine serum albumin: BSA;1-ethyl-3-(3-dimethylaminopropyl)carbodiimide: (EDC;, N-hydroxysuccinimide: NHS.

更新日期：2019-11-20

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