As one of the widely used anthropogenic food additives, 3-tert-butyl-4-hydroxyanisole (3-BHA) has been found to perturb adipogenesis in vitro and induce lipid accumulation in some strains of oleaginous microalgae. The impact of this chemical on adipocyte development and lipid metabolism in mammals remains to be elucidated. In this study, we performed 18-week oral administration of 3-BHA to male C57BL/6J mice with normal diet (ND) or high-fat diet (HFD) and investigated its impacts on adipogenesis and lipid accumulation in vivo. The results indicated that long-term exposure to 3-BHA impacted the mouse body weight gain, white adipose tissue accumulation, and plasma lipids through transcriptional regulation of adipogenesis, lipid metabolism, and adipocyte endocrine function, while glucose metabolism and insulin sensitivity remained unaffected. HFD-fed mice responded to 3-BHA stimulation differently from ND-fed animals, suggesting potential risks for the human burden of 3-BHA in lean and obese subjects. The findings herein validate 3-BHA as an environmental obesogen, and more caution is recommended for its authorized use as a food antioxidant against lipid rancidity.

中文翻译：

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正常和高脂饮食的雄性小鼠脂肪形成中的3-叔丁基-4-羟基茴香醚的摄动。

作为广泛使用的人为食品添加剂之一，已发现3-叔丁基-4-羟基茴香醚（3-BHA）会在体外干扰脂肪形成并在某些油质微藻菌株中诱导脂质蓄积。该化学物质对哺乳动物脂肪细胞发育和脂质代谢的影响尚待阐明。在这项研究中，我们对具有正常饮食（ND）或高脂饮食（HFD）的雄性C57BL / 6J小鼠进行了18周的3-BHA口服给药，并研究了其对体内脂肪形成和脂质蓄积的影响。结果表明，长期暴露于3-BHA会通过脂肪形成，脂质代谢和脂肪细胞内分泌功能的转录调控影响小鼠体重增加，白色脂肪组织积累和血浆脂质，而葡萄糖代谢和胰岛素敏感性仍不受影响。由HFD喂养的小鼠对3-BHA刺激的反应与由ND喂养的动物不同，表明在肥胖和肥胖受试者中3-BHA对人类负担的潜在风险。本文的发现证实了3-BHA是一种环境致病原，并且建议谨慎使用其作为抗脂质酸败的食品抗氧化剂。