Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis.,Nature Plants

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Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis.
Nature Plants ( IF 15.8 ) Pub Date : 2019-11-18 , DOI: 10.1038/s41477-019-0546-1
Myung-Hee Kim ₁ , Jieun Jeon _{1,

2} , Seulbee Lee ₁ , Jae Ho Lee _{1,

2} , Lei Gao ₃ , Byung-Hoon Lee ₂ , Jeong Mee Park ₄ , Yun Ju Kim ₁ , June M Kwak ₂

Affiliation

RNA quality control (RQC) and post-transcriptional gene silencing (PTGS) target and degrade aberrant endogenous RNAs and foreign RNAs, contributing to homeostasis of cellular RNAs. In plants, RQC and PTGS compete for foreign and selected endogenous RNAs; however, little is known about the mechanism interconnecting the two pathways. Using a reporter system designed for monitoring PTGS, we revealed that the 26S proteasome subunit RPT2a enhances transgene PTGS by promoting the accumulation of transgene-derived short interfering RNAs without affecting their biogenesis. RPT2a physically associated with a subset of RQC components and downregulated the protein level. Overexpression of the RQC components interfered with transgene silencing, and impairment of the RQC machinery reinforced transgene PTGS attenuated by rpt2a. Overall, we demonstrate that the 26S proteasome subunit RPT2a promotes PTGS by repressing the RQC machinery to control foreign RNAs.

中文翻译：

蛋白酶体亚基 RPT2a 通过抑制拟南芥中的 RNA 质量控制来促进 PTGS。

RNA 质量控制 (RQC) 和转录后基因沉默 (PTGS) 靶向并降解异常的内源性 RNA 和外源 RNA，有助于细胞 RNA 的稳态。在植物中，RQC 和 PTGS 竞争外源和选定的内源 RNA；然而，关于这两种途径相互连接的机制知之甚少。使用设计用于监测 PTGS 的报告系统，我们发现 26S 蛋白酶体亚基 RPT2a 通过促进转基因衍生的短干扰 RNA 的积累而不影响其生物发生来增强转基因 PTGS。RPT2a 与 RQC 组件的一个子集物理相关并下调蛋白质水平。RQC 成分的过表达干扰了转基因沉默，并且 RQC 机制的损伤增强了 rpt2a 减弱的转基因 PTGS。总体，

更新日期：2019-11-18

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