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Bimetallic metal–organic framework for enzyme immobilization by biomimetic mineralization: constructing a mimic enzyme and simultaneously immobilizing natural enzymes
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.aca.2019.11.039 Junning Wang 1 , Mengyuan Bao 1 , Tianxiang Wei 1 , Zhaoyin Wang 1 , Zhihui Dai 1
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.aca.2019.11.039 Junning Wang 1 , Mengyuan Bao 1 , Tianxiang Wei 1 , Zhaoyin Wang 1 , Zhihui Dai 1
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An iron-nickel bimetallic metal-organic framework (FeNi-MOF) with inner peroxidase-like activity as a support for enzyme immobilization was synthesized for the first time. And the GOx-mediated synthesis of mimic multienzyme system (GOx/FeNi-MOF) was obtained by a one-step biomimetic mineralization. FeNi-MOF played a dual role of a peroxidase mimic and a protective coating. Therefore, the obtained multienzyme system showed both peroxidase-like activity of FeNi-MOF and the biological activity of natural enzyme. FeNi-MOF acted as a peroxidase mimic, showing a higher affinity for hydrogen peroxide (H2O2). The enhanced catalytic activity of the FeNi-MOF may result from synergistic effect between iron and nickel. In addition, the GOx/FeNi-MOF was selected as a proof of concept and succeeded in a one-step colorimetric detection of glucose by tandem catalysis. A small amount of the product was used for glucose detection, and the detection range was 0.3-35 mM, which indicated that our sensor can meet the clinical needs for diagnose diabetes.
中文翻译：
通过仿生矿化固定酶的双金属金属-有机框架：构建模拟酶并同时固定天然酶
首次合成了具有类过氧化物酶活性的铁镍双金属金属有机骨架（FeNi-MOF）作为酶固定化载体。并且通过一步仿生矿化获得了GOx介导的模拟多酶系统（GOx/FeNi-MOF）的合成。FeNi-MOF 具有过氧化物酶模拟物和保护涂层的双重作用。因此，所获得的多酶系统既表现出 FeNi-MOF 的类过氧化物酶活性，又表现出天然酶的生物活性。FeNi-MOF 作为过氧化物酶模拟物，对过氧化氢 (H2O2) 显示出更高的亲和力。FeNi-MOF 的催化活性增强可能是由于铁和镍之间的协同作用。此外，GOx/FeNi-MOF 被选为概念证明，并成功地通过串联催化对葡萄糖进行一步比色检测。少量产品用于葡萄糖检测，检测范围为0.3-35 mM，表明我们的传感器可以满足临床诊断糖尿病的需要。
更新日期：2020-02-01
中文翻译：
通过仿生矿化固定酶的双金属金属-有机框架：构建模拟酶并同时固定天然酶
首次合成了具有类过氧化物酶活性的铁镍双金属金属有机骨架（FeNi-MOF）作为酶固定化载体。并且通过一步仿生矿化获得了GOx介导的模拟多酶系统（GOx/FeNi-MOF）的合成。FeNi-MOF 具有过氧化物酶模拟物和保护涂层的双重作用。因此，所获得的多酶系统既表现出 FeNi-MOF 的类过氧化物酶活性，又表现出天然酶的生物活性。FeNi-MOF 作为过氧化物酶模拟物，对过氧化氢 (H2O2) 显示出更高的亲和力。FeNi-MOF 的催化活性增强可能是由于铁和镍之间的协同作用。此外，GOx/FeNi-MOF 被选为概念证明，并成功地通过串联催化对葡萄糖进行一步比色检测。少量产品用于葡萄糖检测，检测范围为0.3-35 mM，表明我们的传感器可以满足临床诊断糖尿病的需要。
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