Highly active G-quadruplex/hemin DNAzyme for sensitive colorimetric determination of lead(II),Microchimica Acta

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Highly active G-quadruplex/hemin DNAzyme for sensitive colorimetric determination of lead(II)
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-11-15 , DOI: 10.1007/s00604-019-3950-3
Jielin Chen ₁ , Yingying Zhang ₁ , Mingpan Cheng ₁ , Jean-Louis Mergny _{1,

2} , Qianmei Lin ₃ , Jun Zhou ₁ , Huangxian Ju ₁

Affiliation

A UV-vis, CD, and differential pulse voltammetric study was performed on the deactivation of the activity of parallel G-quadruplex/hemin DNAzymes (G4 DNAzymes) by Pb(II). The G4 DNAzyme carries a d[TC] sequence at its 3′ end and is stabilized by potassium(I). On addition of Pb(II), the K(I) ions in the parallel G4 are replaced by Pb(II) to keep the parallel topology. Intruded Pb(II) decrease the affinity between the topology and hemin, this leads to a decrease of DNAzyme activity for catalyzing the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) by hydrogen peroxide to form a green dye with an absorption maximum at 420 nm. The assay does not use any amplification, and has a linear response in the 0.01 to 10 μM Pb(II) concentration range and a 7.1 nM limit of detection. The method was successfully applied to the analysis of spiked water samples. Graphical abstract Schematic diagram of the colorimetric lead(II) assay based on the competition between K+ and Pb2+ stabilized G-quadruplex/hemin DNAzymes (G4 DNAzymes). Schematic diagram of the colorimetric lead(II) assay based on the competition between K+ and Pb2+ stabilized G-quadruplex/hemin DNAzymes (G4 DNAzymes).

中文翻译：

用于灵敏比色测定铅 (II) 的高活性 G-四链体/血红素脱氧核糖核酸酶

对 Pb(II) 对平行 G-四链体/血红素 DNA 酶 (G4 DNA 酶) 活性的失活进行了 UV-vis、CD 和差分脉冲伏安法研究。G4 DNAzyme 在其 3' 末端带有 ad[TC] 序列并由钾 (I) 稳定。添加 Pb(II) 后，平行 G4 中的 K(I) 离子被 Pb(II) 取代以保持平行拓扑。侵入的 Pb(II) 降低了拓扑结构和血红素之间的亲和力，这导致 DNAzyme 催化 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 被过氧化氢氧化形成的 DNAzyme 活性降低在 420 nm 处具有最大吸收的绿色染料。该检测不使用任何扩增，在 0.01 至 10 μM Pb(II) 浓度范围内具有线性响应，检测限为 7.1 nM。该方法已成功应用于加标水样品的分析。图形摘要基于 K+ 和 Pb2+ 稳定的 G-四链体/血红素 DNA 酶 (G4 DNA 酶) 之间竞争的比色铅 (II) 测定示意图。基于 K+ 和 Pb2+ 稳定的 G-四链体/血红素脱氧核糖核酸酶（G4 脱氧核糖核酸酶）之间竞争的比色铅 (II) 分析示意图。

更新日期：2019-11-15

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