A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteria,Microchimica Acta

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A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteria
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-11-15 , DOI: 10.1007/s00604-019-3957-9
Mehrab Pourmadadi ₁ , Javad Shabani Shayeh ₁ , Meisam Omidi ₁ , Fatemeh Yazdian ₂ , Masoud Alebouyeh ₃ , Lobat Tayebi ₄

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An electrochemical aptasensor is described for the voltammetric determination of lipopolysaccharide (LPS) from Escherichia coli 055:B5. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via reduced graphene oxide and gold nanoparticles (RGO/AuNPs). Fast Fourier transform infrared, X-ray diffraction and transmission electron microscopy were used to characterize the nanomaterials. Cyclic voltammetry, square wave voltammetry and electrochemical impedance spectroscopy were used to characterize the modified GCE. The results show that the modified electrode has a good selectivity for LPS over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) is used as an electrochemical probe. The detection limit is 30 fg·mL−1. To decrease the electrochemical potential for detection of LPS, Mg/carbon quantum dots were used as redox active media. They decrease the detection potentialto 0 V and the detection of limit (LOD) to 1 fg·mL−1. The electrode was successfully used to analyze serum of patients and healthy persons. Graphical abstract Schematic representation of the modification of reduced graphene oxide gold nanoparticles with aptamer chains to immobilize on the glassy carbon electrode surface for electrochemical detection of lipopolysaccharides. Schematic representation of the modification of reduced graphene oxide gold nanoparticles with aptamer chains to immobilize on the glassy carbon electrode surface for electrochemical detection of lipopolysaccharides.

中文翻译：

用还原氧化石墨烯和金纳米粒子修饰的玻碳电极用于大肠杆菌脂多糖的电化学适配

描述了一种电化学适体传感器，用于伏安法测定大肠杆菌 055:B5 中的脂多糖 (LPS)。适体链通过还原氧化石墨烯和金纳米粒子 (RGO/AuNPs) 固定在玻璃碳电极 (GCE) 的表面上。快速傅里叶变换红外、X 射线衍射和透射电子显微镜被用来表征纳米材料。循环伏安法、方波伏安法和电化学阻抗谱用于表征改性 GCE。结果表明，与其他生物分子相比，修饰电极对 LPS 具有良好的选择性。六氰基铁酸盐氧化还原系统通常在大约 0.3 V（相对于 Ag/AgCl）下运行，用作电化学探针。检测限为30 fg·mL-1。为了降低检测 LPS 的电化学电位，使用镁/碳量子点作为氧化还原活性介质。它们将检测电位降低至 0 V，将检测限 (LOD) 降低至 1 fg·mL-1。该电极成功用于分析患者和健康人的血清。图形摘要用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。它们将检测电位降低至 0 V，将检测限 (LOD) 降低至 1 fg·mL-1。该电极成功用于分析患者和健康人的血清。图形摘要用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。它们将检测电位降低至 0 V，将检测限 (LOD) 降低至 1 fg·mL-1。该电极成功用于分析患者和健康人的血清。图形摘要用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。图形摘要用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。图形摘要用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。用适体链修饰还原氧化石墨烯金纳米粒子以固定在玻碳电极表面以进行脂多糖电化学检测的示意图。

更新日期：2019-11-15

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