Transcriptional programming and T cell receptor repertoires distinguish human lung and lymph node memory T cells.,Communications Biology

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Transcriptional programming and T cell receptor repertoires distinguish human lung and lymph node memory T cells.
Communications Biology ( IF 5.2 ) Pub Date : 2019-11-13 , DOI: 10.1038/s42003-019-0657-2
Nathan Schoettler _{1,

2} , Cara L Hrusch ₁ , Kelly M Blaine ₁ , Anne I Sperling ₁ , Carole Ober ₂

Affiliation

Antigen-specific memory T cells persist for years after exposure to a pathogen and provide effective recall responses. Many memory T cell subsets have been identified and differ in abundance throughout tissues. This study focused on CD4 and CD8 memory T cells from paired human lung and lung draining lymph node (LDLN) samples and identified substantial differences in the transcriptional landscape of these subsets, including higher expression of an array of innate immune receptors in lung T cells which were further validated by flow cytometry. Using T cell receptor analysis, we determined the clonal overlap between memory T cell subsets within the lung and within the LDLN, and this was greater than the clonal overlap observed between memory T cell subsets compared across tissues. Our results suggest that lung and LDLN memory T cells originate from different precursor pools, recognize distinct antigens and likely have separate roles in immune responses.

中文翻译：

转录编程和 T 细胞受体库可区分人类肺和淋巴结记忆 T 细胞。

抗原特异性记忆 T 细胞在接触病原体后仍能存活数年，并提供有效的回忆反应。许多记忆 T 细胞亚群已被识别，并且在整个组织中的丰度有所不同。这项研究重点关注来自配对人肺和肺引流淋巴结 (LDLN) 样本的 CD4 和 CD8 记忆 T 细胞，并确定了这些亚群转录格局的显着差异，包括肺 T 细胞中一系列先天免疫受体的较高表达，这并通过流式细胞仪进一步验证。通过 T 细胞受体分析，我们确定了肺内和 LDLN 内记忆 T 细胞亚群之间的克隆重叠，这大于跨组织比较的记忆 T 细胞亚群之间观察到的克隆重叠。我们的结果表明，肺和 LDLN 记忆 T 细胞起源于不同的前体池，识别不同的抗原，并且可能在免疫反应中发挥不同的作用。

更新日期：2019-11-13

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