Nanomaterial-based photoenzyme with bifunction is considered as promising mimetic enzyme in mimicking physiological processes. However, the synthesized design of the enzyme is still a significant challenge. Herein,CeO₂@MnO₂ core–shell hollow nanosphere was firstly fabricated by in situ growth of MnO₂ nanolayer on the hollow CeO₂ nanosphere. The prepared heterojunction exhibited excellent photoelectrochemical (PEC) performance because their two-phase interface could facilitate electron transfer under illumination. Meanwhile, the heterojunction had glucose oxidase (GOx)-like photoenzyme property and could catalyze the oxidation of glucose in O₂-saturated PBS (pH 7.0) buffer solution at the bias potential of 0.50 V under visible-light irradiation. The result resulted in the decrease of the photocurrent of CeO₂@MnO₂ heterojunction/FTO electrode. Therefore, a novel non-enzymatic PEC sensor was constructed to detect glucose based on the PEC performance and GOx-like photoenzyme property of CeO₂@MnO₂ heterojunction under visible-light irradiation. Under the optimized conditions, the detection limit was down to 0.07 μM. Meanwhile, the proposed PEC sensor possessed acceptable selectivity, reproducibility and stability. Moreover, the non-enzymatic biosensor was successfully applied to detect glucose levels in human serum samples.

具有双功能的基于纳米材料的光酶被认为是模拟生理过程的有前途的模拟酶。然而，酶的合成设计仍然是一项重大挑战。本文首先通过在中空CeO ₂纳米球上原位生长MnO ₂纳米层来制备CeO ₂ @MnO ₂核壳中空纳米球。制备的异质结表现出优异的光电化学性能（PEC），因为它们的两相界面可以促进照明下的电子转移。同时，异质结具有类似葡萄糖氧化酶（GOx）的光酶特性，并且可以催化O ₂中葡萄糖的氧化。可见光照射下在0.50 V偏压下的饱和PBS（pH 7.0）缓冲溶液。结果导致CeO ₂ @MnO ₂异质结/ FTO电极的光电流降低。因此，在可见光照射下，基于CeO ₂ @MnO ₂异质结的PEC性能和GOx样光酶特性，构建了一种新型的非酶促PEC传感器来检测葡萄糖。在优化条件下，检出限降至0.07μM。同时，所提出的PEC传感器具有可接受的选择性，再现性和稳定性。此外，非酶生物传感器已成功应用于检测人血清样品中的葡萄糖水平。