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Antibacterial PEG-Chitosan Hydrogels for Controlled Antibiotic/Protein Delivery
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-11-08 , DOI: 10.1021/acsabm.9b00570
Peeyush Kumar Sharma 1 , Moumita Halder 1 , Udit Srivastava 1 , Yashveer Singh 1
Affiliation  

Bacterial infections impede the wound-healing process, leading to complications such as chronic wounds and ulcerations. Wound dressings have been used in wound management because of the ease of application, ability to cover irregular wound surfaces, and antibacterial characteristics. For efficient wound management, the dressings must also provide the release of growth factors to aid the tissue regeneration process. Injectable PEG-chitosan hydrogels have been developed for the controlled delivery of ciprofloxacin, an antibiotic, and BSA (decoy for macromolecular growth factors) to prevent infections as well as accelerate the healing process. Hydrogels were fabricated by cross-linking chitosan (0.5% w/v) with bifunctional PEG glyoxylic aldehyde (3, 6, 9, and 12% w/v) and characterized for their cross-linking, surface morphology, viscoelastic, injectability, self-healing, and swelling and degradation characteristics. On loading with ciprofloxacin or BSA, hydrogels provided the sustained release of ciprofloxacin for up to 24 h (≥80%) and that of protein for up to 120 h (≥90%). Ciprofloxacin-loaded hydrogels displayed efficient activities (≥80%) against E. coli and the antibacterial activities were sustained for up to 12 h. The live/dead cell assay showed that the loaded hydrogels are bactericidal, and MTT assay (≥80% cells viable) and cell encapsulation studies showed that hydrogels are nontoxic and cytocompatible toward mammalian cells (NIH-3T3). PEG-chitosan hydrogels may find use as injectable device for the controlled antibiotic and growth factor delivery in antibacterial applications.

中文翻译:

用于控制抗生素/蛋白质递送的抗菌 PEG-壳聚糖水凝胶

细菌感染会阻碍伤口愈合过程,导致慢性伤口和溃疡等并发症。伤口敷料因其易于应用、覆盖不规则伤口表面的能力和抗菌特性而被用于伤口处理。为了有效的伤口管理,敷料还必须释放生长因子以帮助组织再生过程。可注射 PEG-壳聚糖水凝胶已被开发用于控制输送环丙沙星、抗生素和 BSA(大分子生长因子的诱饵),以预防感染并加速愈合过程。通过将壳聚糖(0.5% w/v)与双功能 PEG 乙醛醛(3、6、9 和 12% w/v)交联制备水凝胶,并对其交联、表面形态、粘弹性、可注射性、自愈性以及溶胀和降解特性。在加载环丙沙星或 BSA 后,水凝胶可提供长达 24 小时(≥80%)的环丙沙星缓释和长达 120 小时(≥90%)的蛋白质缓释。负载环丙沙星的水凝胶显示出有效的活性 (≥80%)大肠杆菌和抗菌活性持续长达 12 小时。活/死细胞试验表明,加载的水凝胶具有杀菌作用,MTT 试验(≥80% 的细胞存活)和细胞封装研究表明,水凝胶对哺乳动物细胞 (NIH-3T3) 无毒且具有细胞相容性。PEG-壳聚糖水凝胶可用作抗菌应用中控制抗生素和生长因子递送的注射装置。
更新日期:2019-11-08
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