Conservative transcription in three steps visualized in a double-stranded RNA virus.,Nature Structural & Molecular Biology

当前位置： X-MOL 学术 › Nat. Struct. Mol. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Conservative transcription in three steps visualized in a double-stranded RNA virus.
Nature Structural & Molecular Biology ( IF 12.5 ) Pub Date : 2019-11-06 , DOI: 10.1038/s41594-019-0320-0
Yanxiang Cui ₁ , Yinong Zhang _{1,

2,

3} , Kang Zhou ₁ , Jingchen Sun _{2,

3} , Z Hong Zhou _{1,

2}

Affiliation

Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 Å) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses.

中文翻译：

在双链 RNA 病毒中可视化的三步保守转录。

内源性 RNA 转录以呼肠孤病毒科中的双链 RNA (dsRNA) 病毒为特征，该家族以其简单的单壳成员细胞质多角体病毒 (CPV) 为例。由于在转录过程中缺乏 RNA 依赖的 RNA 聚合酶 (RdRp) 中间阶段的原位结构，人们对 RdRp 如何检测环境线索和内部转录状态以启动和协调衣壳内转录物生产的重复循环知之甚少。在这里，我们在 CPV 的七种实验条件下捕获了五种高分辨率 (2.8-3.5 Å) RdRp-RNA 原位结构——代表静止、起始、早期伸长、伸长和流产状态。我们观察到起始状态下的“Y”型初始 RNA 叉和延伸状态下的完整转录泡。这些结构表明从头 RNA 转录涉及状态转换期间的三个主要构象变化。我们的结果支持用于 dsRNA 病毒内源性保守转录的 ouroboros 模型。

更新日期：2019-11-06

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南