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Acoustic resolution photoacoustic microscopy based on microelectromechanical systems scanner.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2019-11-19 , DOI: 10.1002/jbio.201960127
Mohesh Moothanchery 1 , Kapil Dev 1 , Ghayathri Balasundaram 1 , Renzhe Bi 1 , Malini Olivo 1
Affiliation  

Photoacoustic microscopy (PAM) can be classified as optical resolution (OR)‐PAM and acoustic resolution (AR)‐PAM depending on the type of resolution achieved. Using microelectromechanical systems (MEMS) scanner, high‐speed OR‐PAM system was developed earlier. Depth of imaging limits the use of OR‐PAM technology for many preclinical and clinical imaging applications. Here, we demonstrate the use of a high‐speed MEMS scanner for AR‐PAM imaging. Lateral resolution of 84 μm and an axial resolution of 27 μm with ~2.7 mm imaging depth was achieved using a 50 MHz transducer‐based AR‐PAM system. Use of a higher frequency transducer at 75 MHz has further improved the resolution characteristics of the system with a reduction in imaging depth and a lateral resolution of 53 μm and an axial resolution of 18 μm with ~1.8 mm imaging depth was achieved. Using the two‐axis MEMS scanner a 2 × 2 .5 mm2 area was imaged in 3 seconds. The capability of achieving acoustic resolution images using the MEMS scanner makes it beneficial for the development of high‐speed miniaturized systems for deeper tissue imaging.

中文翻译:

基于微机电系统扫描仪的声分辨率光声显微镜。

根据获得的分辨率类型,光声显微镜(PAM)可以分为光学分辨率(OR)-PAM和声学分辨率(AR)-PAM。使用微机电系统(MEMS)扫描仪,较早开发了高速OR‐PAM系统。成像深度限制了OR‐PAM技术在许多临床前和临床成像应用中的使用。在这里,我们演示了如何将高速MEMS扫描仪用于AR‐PAM成像。使用基于传感器的50 MHz AR-PAM系统,横向分辨率为84μm,轴向分辨率为27μm,成像深度约为2.7 mm。在75 MHz处使用更高频率的换能器进一步改善了系统的分辨率特性,同时降低了成像深度,并且横向分辨率为53μm,轴向分辨率为18μm(成像深度约为1.8 mm)。在3秒钟内对2个区域进行了成像。使用MEMS扫描仪获得声学分辨率图像的能力使其有利于开发用于更深层组织成像的高速小型化系统。
更新日期:2019-11-19
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