2,2',3,5',6-Pentachlorobiphenyl (PCB-95) is an environmental neurotoxicant. There is accumulated evidence that some neurotoxic effects of PCB-95 are caused by increased spontaneous Ca2+ oscillations in neurons resulting from modifying ryanodine receptors (RyR) in calcium-releasing channels. However, there are large gaps in explaining brain and other developmental malformations on embryonic PCB-95 exposure. In the present study, we address those deficiencies by studying the toxic effects of PCB-95 using zebrafish as an ontogenetic model. To characterize these effects, zebrafish embryos with intact chorions were exposed to 4 different concentrations of PCB-95 (0.25, 0.5, 0.75, and 1 ppm) for 3 consecutive days. The controls were maintained in 0.5 × E2 medium or egg water and in 0.1% (v/v) dimethyl sulfoxide (DMSO)/0.5 × E2 medium or egg water. PCB-95-treated groups showed dose-dependent decreases in survival and hatching rates, with increased rates of developmental malformations when compared to controls. These include morphological malformations, brain cell necrosis, and smaller eye sizes at 5 d post fertilization. These data suggest potential mechanisms underlying the abnormal behavior observed in a visual stimulus assay. The present study provides insight into PCB-95-induced developmental toxicity and supports the use of the zebrafish model in understanding the effects of PCB-95 exposure. Environ Toxicol Chem 2019;39:162-170. © 2019 SETAC.

中文翻译：

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胚胎暴露于2,2'，3,5'，6-五氯联苯（PCB-95）会导致斑马鱼发育畸形。

2,2'，3,5'，6-五氯联苯（PCB-95）是一种环境神经毒剂。积累的证据表明，PCB-95的某些神经毒性作用是由于修饰钙释放通道中的ryanodine受体（RyR）导致神经元中自发性Ca2 +振荡增加所致。但是，在解释PCB-95暴露对大脑和其他发育畸形的解释方面仍有很大差距。在本研究中，我们通过使用斑马鱼作为个体发育模型研究PCB-95的毒性作用来解决这些缺陷。为了表征这些影响，将具有完整绒毛膜的斑马鱼胚胎连续4天暴露于4种不同浓度的PCB-95（0.25、0.5、0.75和1 ppm）。将对照保持在0.5×E2培养基或鸡蛋水中，并保持在0.1％（v / v）的二甲基亚砜（DMSO）/ 0中。5×E2培养基或鸡蛋水。与对照组相比，PCB-95治疗组的存活率和孵化率呈剂量依赖性降低，发育畸形率增加。这些包括受精后5 d的形态畸形，脑细胞坏死和较小的眼睛。这些数据表明了潜在的机制，在视觉刺激试验中观察到的异常行为。本研究提供了对PCB-95诱导的发育毒性的见识，并支持使用斑马鱼模型来了解PCB-95暴露的影响。Environ Toxicol Chem 2019; 39：162-170。©2019 SETAC。受精后5 d脑细胞坏死，眼睛变小。这些数据表明了潜在的机制，在视觉刺激试验中观察到的异常行为。本研究提供了对PCB-95诱导的发育毒性的见识，并支持使用斑马鱼模型来了解PCB-95暴露的影响。Environ Toxicol Chem 2019; 39：162-170。©2019 SETAC。受精后5 d脑细胞坏死，眼睛变小。这些数据表明了潜在的机制，在视觉刺激试验中观察到的异常行为。本研究提供了对PCB-95诱导的发育毒性的见识，并支持使用斑马鱼模型来了解PCB-95暴露的影响。Environ Toxicol Chem 2019; 39：162-170。©2019 SETAC。