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Biomimetic 3D DNA Nanomachine via Free DNA Walker Movement on Lipid Bilayers Supported by Hard SiO2@CdTe Nanoparticles for Ultrasensitive MicroRNA Detection
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-11-13 00:00:00 , DOI: 10.1021/acs.analchem.9b03263 Xin Peng 1 , Zhi-Bin Wen 1 , Peng Yang 1 , Ya-Qin Chai 1 , Wen-Bin Liang 1 , Ruo Yuan 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-11-13 00:00:00 , DOI: 10.1021/acs.analchem.9b03263 Xin Peng 1 , Zhi-Bin Wen 1 , Peng Yang 1 , Ya-Qin Chai 1 , Wen-Bin Liang 1 , Ruo Yuan 1
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Herein, a novel three-dimensional (3D) DNA nanomachine with high walking efficiency via free DNA walker movement on biomimetic lipid bilayers supported by hard silica@CdTe quantum dots (SiO2@CdTe) was constructed for ultrasensitive fluorescence detection of microRNA. The synthesized SiO2@CdTe nanoparticles were adopted as the fluorescence indicator and spherical carrier of lipid bilayers, and then the DNA substrates were anchored on lipid bilayers with biomimetic fluidity through the cholesterol-lipid interaction. Once target microRNA-141 interacted with the 3D DNA nanomachine to release cholesterol labeled arm (Chol-arm), the Chol-arm could generate a series of strand displacement reactions by moving freely on the lipid bilayers, resulting in the releasement of numerous quenchers from the SiO2@CdTe nanoparticles and inducing a strong fluorescence signal. Impressively, compared with traditional 3D DNA nanomachine conjugating DNA substrates on hard surfaces (such as gold or silica) with limited reactivity, the proposed biomimetic 3D DNA nanomachine not only immobilized DNA substrates rapidly and effectively but also kept it with a favorable fluidity, which significantly enhanced the walking efficiency. As expected, the biomimetic 3D DNA nanomachine for fluorescence detection of microRNA-141 exhibited an excellent performance with a detection limit of 0.21 pM and presented promising properties in cell lysate detection and intracellular imaging. Thus, the described biomimetic 3D DNA nanomachine provided a novel avenue for sensitive detection of biomolecules, which could be useful for bioanalysis and early clinical diagnoses of disease.
中文翻译：
通过在硬SiO2 @ CdTe纳米颗粒支持的脂质双层上的自由DNA Walker运动实现仿生3D DNA纳米机的超灵敏MicroRNA检测
在此，构建了一种新型的三维（3D）DNA纳米机器，该机器通过在由硬质硅胶@CdTe量子点（SiO 2 @CdTe）支撑的仿生脂质双层上的自由DNA步行者运动而具有高步行效率的高步行效率，用于microRNA的超灵敏荧光检测。合成SiO 2将@CdTe纳米颗粒用作脂质双层的荧光指示剂和球形载体，然后通过胆固醇-脂质相互作用以类似生物的流动性将DNA底物锚定在脂质双层上。一旦靶标microRNA-141与3D DNA纳米机相互作用以释放胆固醇标记的臂（Chol-arm），Chol臂可通过在脂质双层上自由移动而产生一系列链置换反应，从而导致大量淬灭剂从SiO 2@CdTe纳米粒子，并诱导强烈的荧光信号。令人印象深刻的是，与传统的3D DNA纳米机在有限的反应性下将DNA底物缀合在硬质表面（如金或二氧化硅）上相比，拟议的仿生3D DNA纳米机不仅快速有效地固定了DNA底物，而且保持了良好的流动性，这显着提高了步行效率。如预期的那样，用于microRNA-141荧光检测的仿生3D DNA纳米机表现出出色的性能，检测极限为0.21 pM，并且在细胞裂解物检测和细胞内成像中表现出令人鼓舞的特性。因此，所描述的仿生3D DNA纳米机器为生物分子的灵敏检测提供了一条新途径，该途径可用于疾病的生物分析和早期临床诊断。
更新日期：2019-11-13
中文翻译：
通过在硬SiO2 @ CdTe纳米颗粒支持的脂质双层上的自由DNA Walker运动实现仿生3D DNA纳米机的超灵敏MicroRNA检测
在此，构建了一种新型的三维（3D）DNA纳米机器，该机器通过在由硬质硅胶@CdTe量子点（SiO 2 @CdTe）支撑的仿生脂质双层上的自由DNA步行者运动而具有高步行效率的高步行效率，用于microRNA的超灵敏荧光检测。合成SiO 2将@CdTe纳米颗粒用作脂质双层的荧光指示剂和球形载体，然后通过胆固醇-脂质相互作用以类似生物的流动性将DNA底物锚定在脂质双层上。一旦靶标microRNA-141与3D DNA纳米机相互作用以释放胆固醇标记的臂（Chol-arm），Chol臂可通过在脂质双层上自由移动而产生一系列链置换反应，从而导致大量淬灭剂从SiO 2@CdTe纳米粒子，并诱导强烈的荧光信号。令人印象深刻的是，与传统的3D DNA纳米机在有限的反应性下将DNA底物缀合在硬质表面（如金或二氧化硅）上相比，拟议的仿生3D DNA纳米机不仅快速有效地固定了DNA底物，而且保持了良好的流动性，这显着提高了步行效率。如预期的那样，用于microRNA-141荧光检测的仿生3D DNA纳米机表现出出色的性能，检测极限为0.21 pM，并且在细胞裂解物检测和细胞内成像中表现出令人鼓舞的特性。因此，所描述的仿生3D DNA纳米机器为生物分子的灵敏检测提供了一条新途径，该途径可用于疾病的生物分析和早期临床诊断。
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