C3N4 nanosheet-supported Prussian Blue nanoparticles as a peroxidase mimic: colorimetric enzymatic determination of lactate,Microchimica Acta

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C3N4 nanosheet-supported Prussian Blue nanoparticles as a peroxidase mimic: colorimetric enzymatic determination of lactate
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-10-31 , DOI: 10.1007/s00604-019-3834-6
Dandan Zhou ₁ , Congsen Wang ₁ , Junjun Luo ₁ , Minghui Yang ₁

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Prussian Blue nanoparticles were deposited on g-C3N4 nanosheets. The resulting nanocomposite possesses peroxidase-like (POx) activity and can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine at room temperature in the presence of H2O2. This leads to formation of a blue product with an absorption maximum at 650 nm. The formation of the Prussian Blue nanoparticles on the g-C3N4 nanosheets, and the POx-like activity of the composite were characterized in detail. The POx mimic was used for determination of L-lactic acid via detection of H2O2 that is produced by the enzyme lactate oxidase (LOx). The assay has a linear range that extends from 5 to 100 μM, and the detection limit is 2.2 μM. The method was successfully applied to the determination of L-lactic acid in spiked human serum. Graphical abstract Ultra-small Prussian Blue (PB) nanoparticles were used to modify g-C3N4 nanosheets, and their peroxidase-like activity was explored for detection of L-lactic acid. LOx represent L-lactate oxidase, and TMB represents 3,3′,5,5′-tetramethylbenzidine. Ultra-small Prussian Blue (PB) nanoparticles were used to modify g-C3N4 nanosheets, and their peroxidase-like activity was explored for detection of L-lactic acid. LOx represent L-lactate oxidase, and TMB represents 3,3′,5,5′-tetramethylbenzidine.

中文翻译：

C3N4 纳米片支撑的普鲁士蓝纳米粒子作为过氧化物酶模拟物：乳酸的比色酶法测定

普鲁士蓝纳米颗粒沉积在 g-C3N4 纳米片上。所得纳米复合材料具有过氧化物酶样 (POx) 活性，可在室温下在 H2O2 存在下催化 3,3',5,5'-四甲基联苯胺的氧化。这导致形成在 650 nm 处具有最大吸收的蓝色产物。详细表征了 g-C3N4 纳米片上普鲁士蓝纳米颗粒的形成，以及复合材料的类 POx 活性。POx 模拟物用于通过检测乳酸氧化酶 (LOx) 产生的 H2O2 来测定 L-乳酸。该测定具有从 5 到 100 μM 的线性范围，检测限为 2.2 μM。该方法已成功应用于加标人血清中L-乳酸的测定。图形摘要超小普鲁士蓝 (PB) 纳米粒子用于修饰 g-C3N4 纳米片，并探索其过氧化物酶样活性以检测 L-乳酸。LOx 代表 L-乳酸氧化酶，TMB 代表 3,3',5,5'-四甲基联苯胺。超小普鲁士蓝 (PB) 纳米粒子用于修饰 g-C3N4 纳米片，并探索其过氧化物酶样活性以检测 L-乳酸。LOx 代表 L-乳酸氧化酶，TMB 代表 3,3',5,5'-四甲基联苯胺。并探索了它们的过氧化物酶样活性以检测 L-乳酸。LOx 代表 L-乳酸氧化酶，TMB 代表 3,3',5,5'-四甲基联苯胺。并探索了它们的过氧化物酶样活性以检测 L-乳酸。LOx 代表 L-乳酸氧化酶，TMB 代表 3,3',5,5'-四甲基联苯胺。

更新日期：2019-10-31

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