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Simultaneous High-Resolution Detection of Bioenergetic Molecules using Biomimetic-Receptor Nanopore
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-11-11 , DOI: 10.1021/acs.analchem.9b04268
Zhuoqun Su 1 , Yongfeng Wei 1 , Xiao-feng Kang 1
Affiliation  

A novel artificial receptor, heptakis-[6-deoxy-6-(2-hydroxy-3-trimethylammonion-propyl) amino]-beta-cyclomaltoheptaose, with similar functions of mitochondrial ADP/ATP carrier protein, was synthesized and harbored in the engineered α-HL (M113R)7 nanopore, forming a single-molecule biosensor for sensing bioenergetic molecules and their transformations. The strategy significantly elevates both selectivity and signal-to-noise, which enables simultaneous recognition and detection of ATP, ADP, and AMP by real-time single-molecule measurement.

中文翻译:

使用仿生受体纳米孔同时高分辨率检测生物能分子

合成了一种具有线粒体ADP / ATP载体蛋白功能相似的新型人工受体庚七-[6-脱氧-6-(2-羟基-3-三甲基铵-丙基)氨基]-β-环麦芽七糖,并在工程中进行了修饰。 α-HL(M113R)7纳米孔,形成了一个单分子生物传感器,用于感应生物能分子及其转化。该策略显着提高了选择性和信噪比,从而可以通过实时单分子测量同时识别和检测ATP,ADP和AMP。
更新日期:2019-11-11
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