Broad-spectrum resistance to bacterial blight in rice using genome editing.,Nature Biotechnology

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Broad-spectrum resistance to bacterial blight in rice using genome editing.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2019-10-28 , DOI: 10.1038/s41587-019-0267-z
Ricardo Oliva ₁ , Chonghui Ji ₂ , Genelou Atienza-Grande _{1,

3} , José C Huguet-Tapia ₄ , Alvaro Perez-Quintero _{5,

6} , Ting Li ₇ , Joon-Seob Eom ₈ , Chenhao Li ₂ , Hanna Nguyen ₁ , Bo Liu ₂ , Florence Auguy ₅ , Coline Sciallano ₅ , Van T Luu ₈ , Gerbert S Dossa ₉ , Sébastien Cunnac ₅ , Sarah M Schmidt ₈ , Inez H Slamet-Loedin ₁ , Casiana Vera Cruz ₁ , Boris Szurek ₅ , Wolf B Frommer _{8,

10} , Frank F White ₄ , Bing Yang _{2,

11}

Affiliation

International Rice Research Institute, Metro Manila, Philippines.
Division of Plant Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.
College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Philippines.
Department of Plant Pathology, University of Florida, Gainesville, FL, USA.
IRD, CIRAD, Université Montpellier, IPME, Montpellier, France.
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA.
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine Universität Düsseldorf and Max Planck Institute for Plant Breeding Research, Köln, Germany.
Erfurt University of Applied Sciences, Erfurt, Germany.
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Aichi, Japan.
Donald Danforth Plant Science Center, St. Louis, MO, USA.

Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR-Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two TALes from an African Xoo lineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-edited SWEET promoters endow rice lines with robust, broad-spectrum resistance.

中文翻译：

使用基因组编辑对水稻白叶枯病进行广谱抗性。

水稻白叶枯病是亚洲和非洲的重要病害。病原体为米黄单胞菌 (Xanthomonas oryzae pv.)。 oryzae (Xoo) 分泌六种已知转录激活因子样效应子 (TALes) 中的一种或多种，这些效应子结合特定的启动子序列并至少诱导三种宿主蔗糖转运蛋白基因 SWEET11、SWEET13 和 SWEET14 之一，其表达是疾病易感性所必需的。我们使用 CRISPR-Cas9 介导的基因组编辑在所有三个 SWEET 基因启动子中引入突变。通过对 63 个 Xoo 菌株中 TALe 基因的序列分析进一步了解编辑情况，该分析揭示了 SWEET13 等位基因的多个 TALe 变体。 SWEET14 中也产生了突变，这也是来自非洲 Xoo 谱系的两个 TAL 的目标。总共五个启动子突变被同时引入水稻品系 Kitaake 和优良超级品种 IR64 和 Ciherang-Sub1 中。水稻试验表明，基因组编辑的 SWEET 启动子赋予水稻品系强大的广谱抗性。

更新日期：2019-10-28

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