Engineered Ammonia Lyases for the Production of Challenging Electron-Rich l-Phenylalanines,ACS Catalysis

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Engineered Ammonia Lyases for the Production of Challenging Electron-Rich l-Phenylalanines
ACS Catalysis ( IF 11.3 ) Pub Date : 2018-03-07 00:00:00 , DOI: 10.1021/acscatal.8b00496
Syed T. Ahmed ₁ , Fabio Parmeggiani ₁ , Nicholas J. Weise ₁ , Sabine L. Flitsch ₁ , Nicholas J. Turner ₁

Affiliation

Engineered variants of phenylalanine ammonia lyase from Planctomyces brasiliensis were developed through rational design efforts focusing on the aryl binding pocket of the active site, guided by structural and phylogenetic inference. Inherent problems traditionally associated with the biocatalytic hydroamination of acrylic acids, such as low conversion and poor regioselectivity with alkyl and methoxy derivatives, could be overcome. The PbPAL variants described here represent a valuable addition to the biocatalytic toolbox, allowing previously inaccessible amino acid building blocks to be obtained regio- and enantioselectively on preparative scale.

中文翻译：

工程氨水解酶用于生产具有挑战性的富电子的1-苯丙氨酸

通过合理的设计工作，在结构和系统发育推断的指导下，通过合理的设计工作，开发了来自巴西扁平酵母的苯丙氨酸氨裂合酶的工程变体。可以克服传统上与丙烯酸的生物催化加氢胺化相关的固有问题，例如低转化率和烷基和甲氧基衍生物的区域选择性差。本文所述的PbPAL变体代表了生物催化工具箱的宝贵补充，使以前无法获得的氨基酸构造基团可以在制备规模上区域和对映选择性地获得。

更新日期：2018-03-07

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