Baeyer–Villiger Monooxygenases: Tunable Oxidative Biocatalysts,ACS Catalysis

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Baeyer–Villiger Monooxygenases: Tunable Oxidative Biocatalysts
ACS Catalysis ( IF 11.3 ) Pub Date : 2019-11-11 , DOI: 10.1021/acscatal.9b03396
Maximilian J. L. J. Fürst ₁ , Alejandro Gran-Scheuch _{1,

2} , Friso S. Aalbers ₁ , Marco W. Fraaije ₁

Affiliation

Pollution, accidents, and misinformation have earned the pharmaceutical and chemical industry a poor public reputation, despite their undisputable importance to society. Biotechnological advances hold the promise to enable a future of drastically reduced environmental impact and rigorously more efficient production routes at the same time. This is exemplified in the Baeyer–Villiger reaction, which offers a simple synthetic route to oxidize ketones to esters, but application is hampered by the requirement of hazardous and dangerous reagents. As an attractive alternative, flavin-containing Baeyer–Villiger monooxygenases (BVMOs) have been investigated for their potential as biocatalysts for a long time, and many variants have been characterized. After a general look at the state of biotechnology, we here summarize the literature on biochemical characterizations, mechanistic and structural investigations, as well as enzyme engineering efforts in BVMOs. With a focus on recent developments, we critically outline the advances toward tuning these enzymes suitable for industrial applications.

中文翻译：

Baeyer–Villiger单加氧酶：可调节的氧化生物催化剂

尽管污染，事故和错误信息对社会具有无可争议的重要性，但它们在制药和化学工业中赢得了不良的公众声誉。生物技术的进步有望使未来的环境影响大大降低，同时生产路线也更加严格有效。这在Baeyer-Villiger反应中得到了证明，该反应提供了将酮氧化为酯的简单合成路线，但是由于危险和危险试剂的需要而阻碍了其应用。作为一种有吸引力的替代品，长期以来已对含黄素的Baeyer-Villiger单加氧酶（BVMOs）作为生物催化剂的潜力进行了研究，并对许多变体进行了表征。在大致了解生物技术的状态之后，我们在这里总结了BVMOs中有关生化特性，机理和结构研究以及酶工程研究的文献。着眼于最近的发展，我们批判性地概述了调节这些适合工业应用的酶的进展。

更新日期：2019-11-11

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