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Green fluorescent carbon quantum dots functionalized with polyethyleneimine, and their application to aptamer-based determination of thrombin and ATP
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-10-25 , DOI: 10.1007/s00604-019-3874-y Ying Guo 1 , Juncai Zhang 1 , Weihong Zhang 1 , Daodao Hu 2
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-10-25 , DOI: 10.1007/s00604-019-3874-y Ying Guo 1 , Juncai Zhang 1 , Weihong Zhang 1 , Daodao Hu 2
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Brightly fluorescent carbon quantum dots coated with polyethylenimine (PEI-CDs) were prepared using malic acid and PEI as the precursors. The PEI-CDs have a high quantum yield (41%) and green emission (peaking at 502 nm under 430 nm excitation), both of which are not affected by high ionic strength. The PEI-CDs have a positive charge at physiological pH values and can electrostatically bind aptamers with their negative charge. This is shown for aptamers binding thrombin or ATP. Binding of aptamers results in quenching of fluorescence. If thrombin or ATP are introduced, the respective aptamer will bind them, and the complex is then released from the PEI-CDs. Fluorescence increases in proportion to the analyte concentration. Under optimized conditions, thrombin and ATP can be sensitively and selectively detected by fluorometry with lower detection limits of 1.2 and 13 nM, respectively. The assay was successfully applied to the determination of thrombin and of ATP in spiked serum samples. Graphical abstract Green fluorescent carbon quantum dots were functionalized with polyethyleneimine. They were applied to aptamer-based determination of thrombin and ATP. The PEI-functionalized carbon quantum dots (PEI-CDs) have bright green fluorescence are were synthesized by one-step hydrothermal treatment of malic acid and PEI. Employing the PEI-CDs, a fluorometric aptamer-based assay was developed for the determination of thrombin and ATP. Green fluorescent carbon quantum dots were functionalized with polyethyleneimine. They were applied to aptamer-based determination of thrombin and ATP. The PEI-functionalized carbon quantum dots (PEI-CDs) have bright green fluorescence are were synthesized by one-step hydrothermal treatment of malic acid and PEI. Employing the PEI-CDs, a fluorometric aptamer-based assay was developed for the determination of thrombin and ATP.
中文翻译：
聚乙烯亚胺功能化的绿色荧光碳量子点及其在基于适配体测定凝血酶和 ATP 中的应用
使用苹果酸和PEI作为前体制备了涂有聚乙烯亚胺（PEI-CD）的明亮荧光碳量子点。PEI-CD 具有高量子产率 (41%) 和绿色发射（在 430 nm 激发下在 502 nm 处达到峰值），两者都不受高离子强度的影响。PEI-CD 在生理 pH 值下具有正电荷，并且可以静电结合带有负电荷的适配体。这显示了适体结合凝血酶或 ATP。适体的结合导致荧光猝灭。如果引入凝血酶或 ATP，相应的适体将结合它们，然后复合物从 PEI-CD 中释放出来。荧光与分析物浓度成正比。在优化条件下，凝血酶和 ATP 可以通过荧光法灵敏和选择性地检测到，检测限分别为 1.2 和 13 nM。该测定已成功应用于测定加标血清样品中的凝血酶和 ATP。图形摘要绿色荧光碳量子点用聚乙烯亚胺功能化。它们被应用于基于适配体的凝血酶和 ATP 测定。PEI 功能化碳量子点 (PEI-CDs) 具有亮绿色荧光是通过苹果酸和 PEI 一步水热处理合成的。使用 PEI-CD，开发了一种基于荧光适体的测定法，用于测定凝血酶和 ATP。绿色荧光碳量子点用聚乙烯亚胺功能化。它们被应用于基于适配体的凝血酶和 ATP 测定。PEI 功能化碳量子点 (PEI-CDs) 具有亮绿色荧光是通过苹果酸和 PEI 一步水热处理合成的。使用 PEI-CD，开发了一种基于荧光适体的测定法，用于测定凝血酶和 ATP。
更新日期：2019-10-25
中文翻译：
聚乙烯亚胺功能化的绿色荧光碳量子点及其在基于适配体测定凝血酶和 ATP 中的应用
使用苹果酸和PEI作为前体制备了涂有聚乙烯亚胺（PEI-CD）的明亮荧光碳量子点。PEI-CD 具有高量子产率 (41%) 和绿色发射（在 430 nm 激发下在 502 nm 处达到峰值），两者都不受高离子强度的影响。PEI-CD 在生理 pH 值下具有正电荷，并且可以静电结合带有负电荷的适配体。这显示了适体结合凝血酶或 ATP。适体的结合导致荧光猝灭。如果引入凝血酶或 ATP，相应的适体将结合它们，然后复合物从 PEI-CD 中释放出来。荧光与分析物浓度成正比。在优化条件下，凝血酶和 ATP 可以通过荧光法灵敏和选择性地检测到，检测限分别为 1.2 和 13 nM。该测定已成功应用于测定加标血清样品中的凝血酶和 ATP。图形摘要绿色荧光碳量子点用聚乙烯亚胺功能化。它们被应用于基于适配体的凝血酶和 ATP 测定。PEI 功能化碳量子点 (PEI-CDs) 具有亮绿色荧光是通过苹果酸和 PEI 一步水热处理合成的。使用 PEI-CD，开发了一种基于荧光适体的测定法，用于测定凝血酶和 ATP。绿色荧光碳量子点用聚乙烯亚胺功能化。它们被应用于基于适配体的凝血酶和 ATP 测定。PEI 功能化碳量子点 (PEI-CDs) 具有亮绿色荧光是通过苹果酸和 PEI 一步水热处理合成的。使用 PEI-CD，开发了一种基于荧光适体的测定法，用于测定凝血酶和 ATP。
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