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Yolk-shell structured Au@Ag@mSiO2 as a probe for sensing cysteine enantiomers and Cu2+ based on circular dichroism.
Analyst ( IF 3.6 ) Pub Date : 2019-10-23 , DOI: 10.1039/c9an01541h Jing Wang 1 , Xu Xu , Xiaolin Qiu , Shuaishuai Zhang , Yinxian Peng
Analyst ( IF 3.6 ) Pub Date : 2019-10-23 , DOI: 10.1039/c9an01541h Jing Wang 1 , Xu Xu , Xiaolin Qiu , Shuaishuai Zhang , Yinxian Peng
Affiliation
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A yolk–shell structured Au@Ag@mSiO2 probe was fabricated by coating a layer of mesoporous silica on the surface of a Au@Ag core–shell nanosphere, followed by partially removing the Ag shell. Mirror symmetrical chiral signals at ∼258 nm in the UV region were observed for the probe upon coupling with cysteine enantiomers on the surface of Au@Ag. The intensity of the CD signal of the probe is enhanced by increasing L/D-Cys concentration, allowing the quantitative determination of the cysteine enantiomers. The developed method shows an excellent linear relationship between the CD signal and L-Cys concentration ranging from 10 μM to 90 μM with a limit of detection of 8.5 μM. In the presence of Cu2+, the CD signal of the probe weakened due to the oxidation of L-cysteine to L-cystine catalyzed by Cu2+. Based on this phenomenon, a new strategy for the detection of Cu2+ can be developed. Under the optimized conditions, the CD signal decreases linearly with the log of the concentration of Cu2+ in the range from 1 to 250 nM with a detection limit of 0.1 nM.
中文翻译：
卵黄结构的Au @ Ag @ mSiO2作为探针，基于圆二色性检测半胱氨酸对映体和Cu2 +。
卵黄壳结构的Au @ Ag @ mSiO 2探针是通过在Au @ Ag核壳纳米球的表面涂覆一层介孔二氧化硅，然后部分去除Ag壳而制成的。与Au @ Ag表面上的半胱氨酸对映体偶合后，在紫外区的约258 nm处观察到镜面对称手性信号。通过增加L / D -Cys浓度可增强探针CD信号的强度，从而可以定量测定半胱氨酸对映体。所开发的方法显示CD信号与L -Cys浓度在10μM至90μM之间具有极好的线性关系，检出限为8.5μM。在Cu 2+存在下，由于Cu 2+催化L-半胱氨酸氧化为L-胱氨酸，探针的CD信号减弱。基于这种现象，可以开发出一种检测Cu 2+的新策略。在最佳条件下，CD信号随Cu 2+浓度在1至250 nM范围内的对数线性下降，检出限为0.1 nM。
更新日期：2019-10-23
中文翻译：
卵黄结构的Au @ Ag @ mSiO2作为探针，基于圆二色性检测半胱氨酸对映体和Cu2 +。
卵黄壳结构的Au @ Ag @ mSiO 2探针是通过在Au @ Ag核壳纳米球的表面涂覆一层介孔二氧化硅，然后部分去除Ag壳而制成的。与Au @ Ag表面上的半胱氨酸对映体偶合后，在紫外区的约258 nm处观察到镜面对称手性信号。通过增加L / D -Cys浓度可增强探针CD信号的强度，从而可以定量测定半胱氨酸对映体。所开发的方法显示CD信号与L -Cys浓度在10μM至90μM之间具有极好的线性关系，检出限为8.5μM。在Cu 2+存在下，由于Cu 2+催化L-半胱氨酸氧化为L-胱氨酸，探针的CD信号减弱。基于这种现象，可以开发出一种检测Cu 2+的新策略。在最佳条件下，CD信号随Cu 2+浓度在1至250 nM范围内的对数线性下降，检出限为0.1 nM。
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