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Functional characterization of olfactory receptors in three Dacini fruit flies (Diptera: Tephritidae) that respond to 1-nonanol analogs as components in the rectal glands.
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2019-09-06 , DOI: 10.1016/j.cbpb.2019.110346
Hajime Ono 1 , Hitomi Miyazaki 1 , Hidefumi Mitsuno 2 , Katsuhisa Ozaki 3 , Ryohei Kanzaki 2 , Ritsuo Nishida 1
Affiliation  

Dacini fruit flies (Tephritidae: Diptera), including destructive pest species, are strongly affected in their reproductive behaviors by semiochemicals. Notably, male lures have been developed for pest management e.g., aromatic compounds for the Oriental fruit fly Bactrocera dorsalis and the melon fruit fly Zeugodacus cucurbitae; terpenic α-ionone analogs for the solanaceous fruit fly, B. latifrons. Other than those specific male attractants, 1-nonanol analogs have been noticed as major aliphatic components in the male rectal gland, which is considered as a secretory organ of male sex pheromones. Although multiple semiochemicals associated with the life cycle of Dacini fruit flies have been identified, their behavioral role(s) and chemosensory mechanisms by which the perception occurs have not been fully elucidated. In this study, we conducted RNA sequencing analysis of the chemosensory organs of B. latifrons and Z. cucurbitae to identify the genes coding for chemosensory receptors. Because the skeletons of male attractants are different among Dacini fruit fly species, we analyzed phylogenetic relationships of candidate olfactory receptors (ORs) among the three species. We found that the OR phylogeny reflects the taxonomic relationships of the three species. We further characterized functional properties of OR74a in the three Dacini species to the 1-nonanol analogs related to components in the rectal glands. The three OR74a homologs responded to 1-nonanol, but their sensitivities differed from each other. The OR74a homologs identified from B. dorsalis and Z. cucurbitae responded significantly to 6-oxo-1-nonanol, but not to 1,3-nonanediol and nonyl acetate, indicating similar binding properties of the homologous ORs.

中文翻译:

三种达西尼果蝇(双翅目:蝇科)中的嗅觉受体的功能表征,这些果蝇对1-壬醇类似物作为直肠腺体的成分做出反应。

达西尼果蝇(T科:双翅目),包括破坏性害虫,受到化学信息素的生殖行为的强烈影响。值得注意的是,已经开发了用于诱虫管理的雄性诱剂,例如东方果蝇小实蝇和瓜果蝇葫芦科的芳香化合物。茄果蝇B. latifrons的萜烯α-紫罗兰酮类似物。除了那些特定的雄性引诱剂外,人们还注意到1-壬醇类似物是雄性直肠腺体中的主要脂肪族成分,被认为是雄性信息素的分泌器官。尽管已鉴定出与达西尼果蝇的生命周期相关的多种化学信息素,但尚未充分阐明它们的行为作用和发生感知的化学感觉机制。在这项研究中,我们对B. latifrons和Z.葫芦科的化学感觉器官进行了RNA测序分析,以鉴定编码化学感觉受体的基因。由于达西尼果蝇物种中雄性引诱剂的骨架不同,因此我们分析了这三种物种中候选嗅觉受体(OR)的系统发育关系。我们发现,OR系统发育反映了这三个物种的分类学关系。我们进一步表征了在三个Dacini物种中的OR74a的功能特性与与直肠腺体成分相关的1-壬醇类似物。这三个OR74a同源物对1-壬醇有反应,但它们的敏感性互不相同。从桔小实蝇和葫芦科植物鉴定到的OR74a同源物对6-氧代-1-壬醇有明显反应,但对1,3-壬基二醇和乙酸壬酯反应却不明显,
更新日期:2019-09-06
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