A super-resolution platform for correlative live single-molecule imaging and STED microscopy.,Nature Methods

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A super-resolution platform for correlative live single-molecule imaging and STED microscopy.
Nature Methods ( IF 36.1 ) Pub Date : 2019-10-21 , DOI: 10.1038/s41592-019-0611-8
V V G Krishna Inavalli _{1,

2} , Martin O Lenz _{1,

2} , Corey Butler _{1,

2,

3} , Julie Angibaud _{1,

2} , Benjamin Compans _{1,

2} , Florian Levet _{1,

2,

4} , Jan Tønnesen _{1,

2} , Olivier Rossier _{1,

2} , Gregory Giannone _{1,

2} , Olivier Thoumine _{1,

2} , Eric Hosy _{1,

2} , Daniel Choquet _{1,

2,

4} , Jean-Baptiste Sibarita _{1,

2} , U Valentin Nägerl _{1,

2}

Affiliation

Super-resolution microscopy offers tremendous opportunities to unravel the complex and dynamic architecture of living cells. However, current super-resolution microscopes are well suited for revealing protein distributions or cell morphology, but not both. We present a super-resolution platform that permits correlative single-molecule imaging and stimulated emission depletion microscopy in live cells. It gives nanoscale access to the positions and movements of synaptic proteins within the morphological context of growth cones and dendritic spines.

中文翻译：

用于相关实时单分子成像和 STED 显微镜的超分辨率平台。

超分辨率显微镜为揭示活细胞的复杂和动态结构提供了巨大的机会。然而，目前的超分辨率显微镜非常适合揭示蛋白质分布或细胞形态，但不能同时显示两者。我们提出了一个超分辨率平台，允许在活细胞中进行相关的单分子成像和受激发射耗尽显微镜。它提供了在生长锥和树突棘的形态学背景下对突触蛋白的位置和运动进行纳米级访问。

更新日期：2019-10-21

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