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Mannose-Coated Fluorescent Lipid Microparticles for Specific Cellular Targeting and Internalization via Glycoreceptor-Induced Phagocytosis
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-11-04 , DOI: 10.1021/acsabm.9b00793
Blaise Dumat 1 , Lorraine Montel 2, 3 , Léa Pinon 2, 3 , Pascal Matton 1 , Laurent Cattiaux 1 , Jacques Fattaccioli 2, 3 , Jean-Maurice Mallet 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-11-04 , DOI: 10.1021/acsabm.9b00793
Blaise Dumat 1 , Lorraine Montel 2, 3 , Léa Pinon 2, 3 , Pascal Matton 1 , Laurent Cattiaux 1 , Jacques Fattaccioli 2, 3 , Jean-Maurice Mallet 1
Affiliation
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In this work, we report on the development of mannose-coated fluorescent lipid microparticles to study the role of C-type lectin membrane receptors in phagocytosis. The micrometric droplets of soybean oil-in-water emulsion were functionalized with a tailor-made fluorescent mannolipid. The amphiphilic ligand was built from a mannose unit, a lipid C11 spacer, and a naphthalimide fluorophore. The functionalization of the droplets was monitored by fluorescence microscopy as well as their interaction with concanavalin A, which was used as a model lectin in vitro. The use of a monovalent ligand on the surface of emulsion droplets yielded particles with an affinity approximately 40 times higher than that of free mannose. In cellulo, the coated droplets were shown to be specifically internalized by macrophages in a receptor-dependent phagocytic pathway. The naked droplets, on the other hand, displayed very little internalization because of their low immunogenicity. This work thus brings evidence that C-type lectin membrane receptors may act as phagocytic receptors. The functionalization of the droplets with the tailored amphiphilic fluorescent ligand also provides insights into the development of organic fluorescent particles that may prove useful for developing targeted imaging and delivery tools.
中文翻译：
甘露糖包被的荧光脂质微粒通过糖受体诱导的吞噬作用进行特异性细胞靶向和内化
在这项工作中，我们报告了甘露糖涂层荧光脂质微粒的发展，以研究 C 型凝集素膜受体在吞噬作用中的作用。用特制的荧光甘露脂对水包油乳液的微米级液滴进行功能化。两亲配体由甘露糖单元、脂质 C11 间隔物和萘酰亚胺荧光团构成。通过荧光显微镜监测液滴的功能化以及它们与刀豆球蛋白 A 的相互作用，刀豆球蛋白 A 被用作体外凝集素的模型。在乳液液滴表面使用单价配体产生的颗粒的亲和力比游离甘露糖的亲和力高约 40 倍。在蜂窝，涂层液滴被证明在受体依赖性吞噬途径中被巨噬细胞特异性内化。另一方面，裸液滴由于其低免疫原性而显示出非常少的内化。因此，这项工作提供了 C 型凝集素膜受体可能充当吞噬受体的证据。用定制的两亲荧光配体对液滴进行功能化还提供了对有机荧光颗粒开发的见解，这可能对开发靶向成像和递送工具有用。
更新日期：2019-11-04
中文翻译：
甘露糖包被的荧光脂质微粒通过糖受体诱导的吞噬作用进行特异性细胞靶向和内化
在这项工作中，我们报告了甘露糖涂层荧光脂质微粒的发展，以研究 C 型凝集素膜受体在吞噬作用中的作用。用特制的荧光甘露脂对水包油乳液的微米级液滴进行功能化。两亲配体由甘露糖单元、脂质 C11 间隔物和萘酰亚胺荧光团构成。通过荧光显微镜监测液滴的功能化以及它们与刀豆球蛋白 A 的相互作用，刀豆球蛋白 A 被用作体外凝集素的模型。在乳液液滴表面使用单价配体产生的颗粒的亲和力比游离甘露糖的亲和力高约 40 倍。在蜂窝，涂层液滴被证明在受体依赖性吞噬途径中被巨噬细胞特异性内化。另一方面，裸液滴由于其低免疫原性而显示出非常少的内化。因此，这项工作提供了 C 型凝集素膜受体可能充当吞噬受体的证据。用定制的两亲荧光配体对液滴进行功能化还提供了对有机荧光颗粒开发的见解，这可能对开发靶向成像和递送工具有用。
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