Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures.,Cell

当前位置： X-MOL 学术 › Cell › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures.
Cell ( IF 45.5 ) Pub Date : 2019-10-17 , DOI: 10.1016/j.cell.2019.09.029
Ronghui Li ₁ , Cuiqing Zhong ₁ , Yang Yu ₂ , Haisong Liu ₁ , Masahiro Sakurai ₃ , Leqian Yu ₄ , Zheying Min ₅ , Lei Shi ₆ , Yulei Wei ₇ , Yuta Takahashi ₁ , Hsin-Kai Liao ₆ , Jie Qiao ₅ , Hongkui Deng ₈ , Estrella Nuñez-Delicado ₉ , Concepcion Rodriguez Esteban ₁ , Jun Wu ₄ , Juan Carlos Izpisua Belmonte ₁

Affiliation

Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China; Stem Cell Research Center, Peking University Third Hospital, Beijing, 100191, China.
Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.
Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Universidad Catolica, San Antonio de Murcia, Campus de los Jeronimos, 135, Guadalupe 30107, Murcia, Spain.
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China.
University of Peking, 5 Yiheyuan Rd, Haidian Qu, Beijing 100871, China.
Universidad Catolica, San Antonio de Murcia, Campus de los Jeronimos, 135, Guadalupe 30107, Murcia, Spain.

A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to creating viable synthetic embryos by using cultured cells.

中文翻译：

从小鼠胚胎和成年细胞培养物产生的囊胚样结构。

从胚胎到8细胞阶段的单个小鼠卵裂球都可以产生整个胚泡。实验室培养的细胞是否保留相似的生成能力仍然未知。从单一干细胞类型，扩展的多能干（EPS）细胞开始，我们建立了3D分化系统，该系统能够通过谱系分离和自组织生成囊胚样结构（EPS-类胚）。EPS胚泡在形态和细胞谱系分配方面类似于胚泡，并且在体外植入前和植入后早期发育过程中概括了关键的形态发生事件。转移后，一些EPS囊胚进行了植入，蜕膜化，并在子宫内产生了活的，尽管是杂乱的组织。单细胞和大量RNA测序分析表明EPS囊胚包含所有三个囊胚细胞谱系，并且与天然囊胚共享转录相似性。我们还提供了概念证明，即可以通过细胞重编程从成年细胞中产生EPS胚泡。EPS胚泡为研究早期胚胎发生提供了独特的平台，并为通过使用培养的细胞创造可行的合成胚胎铺平了道路。

更新日期：2019-10-17

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南