Experimental characterization of the complete life cycle of Haemoproteus columbae, with a description of a natural host-parasite system used to study this infection.,International Journal for Parasitology

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Experimental characterization of the complete life cycle of Haemoproteus columbae, with a description of a natural host-parasite system used to study this infection.
International Journal for Parasitology ( IF 3.7 ) Pub Date : 2019-10-16 , DOI: 10.1016/j.ijpara.2019.07.003
Axl S Cepeda ₁ , Ingrid A Lotta-Arévalo ₁ , David F Pinto-Osorio ₁ , Jhon Macías-Zacipa ₂ , Gediminas Valkiūnas ₃ , Paola Barato ₄ , Nubia E Matta ₁

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Characterization of complete life cycles of haemoparasites requires the maintenance of suitable susceptible vertebrate hosts and vectors for long periods in captivity, in order to follow the complete parasitic cycle in definitive and intermediate hosts. Currently, there are few host-parasite models established in avian haemosporidian research, and those have been developed mainly for species of Passeriformes and their parasites. This study aimed to develop an experimental methodology to access the complete life cycle of Haemoproteus columbae (cytb lineage HAECOL1), which parasitizes the Rock Pigeon (Columba livia) and louse fly (Pseudolynchia canariensis). A colony of louse flies, which are the natural vectors of this parasite, was established. Thirty newly emerged insects were exposed to H. columbae infection and used to infect naïve Rock Pigeons. The peak of parasitaemia (acute stage) was seen between 27 and 32 days p.i. when up to 70.8% of red blood cells were infected. The crisis occurred approximately 1 week after the peak, and the long-lasting chronic parasitaemia stage followed. Exo-erythrocytic meronts were seen mainly in the lungs where extensive tissue damage was reported, but also in the kidneys and spleen. In the vector, the sporogonic cycle of H. columbae was completed between 13 and 16 days p.i., at an average temperature ranging between 12 and 15 °C. This host-parasite model is tractable for maintenance in captivity. It is recommended for use in studies aiming for detailed characterization of host-parasite relationships in areas such as physiology, pathology, immunobiology, genetics, as well as for evaluative treatments and to follow the infection in any stage of parasite development both in the vertebrate or invertebrate host.

中文翻译：

实验描述了血球菌整个生命周期，并描述了用于研究这种感染的天然宿主-寄生虫系统。

血寄生虫完整生命周期的表征要求长期保持适当的易感脊椎动物宿主和媒介物圈养，以便跟随最终宿主和中间宿主的完整寄生虫周期。目前，在禽血孢病研究中建立的宿主-寄生虫模型很少，而这些模型主要是针对Passeriformes的物种及其寄生虫开发的。这项研究的目的是开发一种实验方法来访问整个血统的整个生命周期（血统世系HAECOL1），该生命周期寄生了原鸽（livia Columba livia）和虱蝇（Pseudolynchia canariensis）。建立了虱蝇的菌落，它们是该寄生虫的天然载体。30只新出现的昆虫受到了哥伦布杆菌的感染，并被用于感染幼稚的原鸽。当感染多达70.8％的红细胞时，在寄生虫血症高峰期（急性期）在pi的27至32天之间可见。危机发生在高峰后约1周，随后出现了长期的慢性寄生虫血症阶段。外红细胞外膜性坏死主要见于据报道广泛组织损伤的肺部，也见于肾脏和脾脏中。在载体中，大肠杆菌的孢子形成周期在pi的13至16天之间完成，平均温度在12至15°C之间。该宿主-寄生虫模型易于维持人工饲养。建议将其用于旨在详细表征诸如生理学，病理学，免疫生物学，遗传学，

更新日期：2019-10-16

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