In the decade since their advent, ablative fractional lasers have emerged as powerful tools to enhance drug delivery to and through the skin. Effective and highly customizable, laser-assisted drug delivery (LADD) has led to improved therapeutic outcomes for several medical indications. However, for LADD to reach maturity as a standard treatment technique, a greater appreciation of its underlying science is needed. This work aims to provide an in-depth guide to the technology's fundamental principles, experimental methodology and unique aspects of LADD data interpretation. We show that drug's physicochemical properties including solubility, molecular weight and tissue binding behavior, are crucial determinants of how laser channel morphology influences topical delivery. Furthermore, we identify strengths and limitations of experimental models and drug detection techniques, interrogating the usefulness of in vitro data in predicting LADD in vivo. By compiling insights from over 75 studies, we ultimately devise an approach for intelligent application of LADD, supporting its implementation in the clinical setting.

自问世以来的十年中，烧蚀点阵激光已成为增强药物输送到皮肤和通过皮肤的强大工具。有效且高度可定制的激光辅助给药 (LADD) 已改善了多种医学适应症的治疗效果。然而，为了使 LADD 作为标准治疗技术成熟，需要对其基础科学有更深入的了解。这项工作旨在为该技术的基本原理、实验方法和 LADD 数据解释的独特方面提供深入的指导。我们表明，药物的理化特性，包括溶解度、分子量和组织结合行为，是激光通道形态如何影响局部给药的关键决定因素。此外，体内预测 LADD 的体外数据。通过汇集超过 75 项研究的见解，我们最终设计了一种智能应用 LADD 的方法，支持其在临床环境中的实施。