当前位置：
X-MOL 学术
›
J. Org. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Solid Supports for the Synthesis of 3'-Aminooxy Deoxy- or Ribo-oligonucleotides and Their 3'-Conjugation by Oxime Ligation.
The Journal of Organic Chemistry ( IF 3.3 ) Pub Date : 2019-10-25 , DOI: 10.1021/acs.joc.9b00848 Mathieu Noël 1 , Céline Clément-Blanc 1 , Albert Meyer 1 , Jean-Jacques Vasseur 1 , François Morvan 1
The Journal of Organic Chemistry ( IF 3.3 ) Pub Date : 2019-10-25 , DOI: 10.1021/acs.joc.9b00848 Mathieu Noël 1 , Céline Clément-Blanc 1 , Albert Meyer 1 , Jean-Jacques Vasseur 1 , François Morvan 1
Affiliation
|
Mono- and triethylene glycol aminooxy derivatives were reacted with levulinic acid, protected with dimethoxytrityl, and immobilized on solid support. The resulting solid supports were used for elongation of oligonucleotides. Then, a mild ammonia treatment was applied to remove the oligonucleotide protecting groups, followed by a treatment with 50 mM methoxyamine at pH 4.2, releasing the 3'-aminooxy oligonucleotides by an oxime exchange reaction. The resulting 3'-aminooxy deoxy- or ribo-oligonucleotides were conjugated to various ketones and aldehydes with high efficiency by oxime ligation.
中文翻译：
用于合成3'-氨基氧基脱氧或核糖寡核苷酸及其通过肟连接的3'-偶联的固体支持物。
使单乙二醇和三乙二醇氨基氧基衍生物与乙酰丙酸反应，用二甲氧基三苯甲基保护，并固定在固体支持物上。所得的固体支持物用于寡核苷酸的延伸。然后，进行温和的氨处理以除去寡核苷酸保护基，然后用50mM的pH 4.2的甲氧基胺处理，通过肟交换反应释放3'-氨基氧基寡核苷酸。通过肟连接，将所得的3'-氨氧基脱氧或核糖寡核苷酸与各种酮和醛高效地偶联。
更新日期：2019-10-25
中文翻译：
用于合成3'-氨基氧基脱氧或核糖寡核苷酸及其通过肟连接的3'-偶联的固体支持物。
使单乙二醇和三乙二醇氨基氧基衍生物与乙酰丙酸反应，用二甲氧基三苯甲基保护，并固定在固体支持物上。所得的固体支持物用于寡核苷酸的延伸。然后，进行温和的氨处理以除去寡核苷酸保护基，然后用50mM的pH 4.2的甲氧基胺处理，通过肟交换反应释放3'-氨基氧基寡核苷酸。通过肟连接，将所得的3'-氨氧基脱氧或核糖寡核苷酸与各种酮和醛高效地偶联。
京公网安备 11010802027423号