赋予盐/干旱和氧化胁迫耐受性的番薯属非典型LEA基因（IpLEA）的分离和鉴定。,Scientific Reports

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赋予盐/干旱和氧化胁迫耐受性的番薯属非典型LEA基因（IpLEA）的分离和鉴定。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-10-16 , DOI: 10.1038/s41598-019-50813-w
Jiexuan Zheng _{1,

2,

3} , Huaxiang Su _{1,

2,

3} , Ruoyi Lin _{1,

2,

4} , Hui Zhang _{1,

2,

3} , Kuaifei Xia _{1,

2} , Shuguang Jian _{1,

2} , Mei Zhang _{1,

2}

Affiliation

晚期胚胎发生丰富（LEA）蛋白属于一个大家族，广泛存在于植物中，主要参与植物发育过程中的干燥过程或对非生物胁迫的响应。在这里，我们报道了一个非典型的LEA基因（IpLEA），它与番薯科的豆科植物的耐盐性有关。序列分析表明IpLEA属于LEA_2（PF03168）组。已显示IpLEA具有细胞质定位模式。定量逆转录PCR分析表明，IpLEA在异荚鱼属植物的不同器官中广泛表达，并且在盐，渗透，氧化，冷冻和脱落酸处理后其表达水平增加。对IpLEA的1,495 bp启动子的分析确定了参与非生物胁迫的独特顺式调控元件。与非生物胁迫下的对照相比，诱导IpLEA改善了大肠杆菌的生长性能。为了进一步评估IpLEA在植物中的功能，产生了过表达IpLEA的转基因拟南芥植物。与野生型相比，过量表达IpLEA的拟南芥幼苗和成年植物对盐和干旱胁迫的耐受性更高。转基因植物还显示出比野生型拟南芥更高的氧化胁迫耐受性。此外，一系列应激反应基因的表达模式受到影响。结果表明，IpLEA可能通过介导水稳态或通过充当活性氧清除剂来参与植物对盐和干旱的反应，从而影响微生物和植物在各种非生物胁迫下的生理过程。

"点击查看英文标题和摘要"

Isolation and characterization of an atypical LEA gene (IpLEA) from Ipomoea pes-caprae conferring salt/drought and oxidative stress tolerance.

Late embryogenesis abundant (LEA) proteins belong to a large family that exists widely in plants and is mainly involved in desiccation processes during plant development or in the response to abiotic stresses. Here, we reported on an atypical LEA gene (IpLEA) related to salt tolerance from Ipomoea pes-caprae L. (Convolvulaceae). Sequence analysis revealed that IpLEA belongs to the LEA_2 (PF03168) group. IpLEA was shown to have a cytoplasmic localization pattern. Quantitative reverse transcription PCR analysis showed that IpLEA was widely expressed in different organs of the I. pes-caprae plants, and the expression levels increased following salt, osmotic, oxidative, freezing, and abscisic acid treatments. Analysis of the 1,495 bp promoter of IpLEA identified distinct cis-acting regulatory elements involved in abiotic stress. Induction of IpLEA improved Escherichia coli growth performance compared with the control under abiotic stresses. To further assess the function of IpLEA in plants, transgenic Arabidopsis plants overexpressing IpLEA were generated. The IpLEA-overexpressing Arabidopsis seedlings and adult plants showed higher tolerance to salt and drought stress than the wild-type. The transgenic plants also showed higher oxidative stress tolerance than the wild-type Arabidopsis. Furthermore, the expression patterns of a series of stress-responsive genes were affected. The results indicate that IpLEA is involved in the plant response to salt and drought, probably by mediating water homeostasis or by acting as a reactive oxygen species scavenger, thereby influencing physiological processes under various abiotic stresses in microorganisms and plants.

更新日期：2019-10-17

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