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Endophyte Bacillus subtilis evade plant defense by producing lantibiotic subtilomycin to mask self-produced flagellin.
Communications Biology ( IF 5.2 ) Pub Date : 2019-10-10 , DOI: 10.1038/s42003-019-0614-0
Yun Deng 1 , Hanqiao Chen 1 , Congzhi Li 1 , Jianyi Xu 1 , Qingdong Qi 1 , Yuanyuan Xu 1 , Yiguang Zhu 1 , Jinshui Zheng 1 , Donghai Peng 1 , Lifang Ruan 1 , Ming Sun 1
Affiliation  

Microbes can enter into healthy plants as endophytes and confer beneficial functions. The entry of commensal microbes into plants involves penetrating plant defense. Most mechanisms about overcoming plant defense are focused on adapted pathogens, while the mechanism involved in beneficial endophyte evades plant defense to achieve harmonious commensalism is unclear. Here, we discover a mechanism that an endophyte bacterium Bacillus subtilis BSn5 reduce to stimulate the plant defensive response by producing lantibiotic subtilomycin to bind self-produced flagellin. Subtilomycin bind with flagellin and affect flg22-induced plant defense, by which means promotes the endophytic colonization in A. thaliana. Subtilomycin also promotes the BSn5 colonization in a distinct plant, Amorphophallus konjac, where the BSn5 was isolated. Our investigation shows more independent subtilomycin/-like producers are isolated from distinct plants. Our work unveils a common strategy that is used for bacterial endophytic colonization.

中文翻译:


内生菌枯草芽孢杆菌通过产生羊毛硫抗生素枯草霉素来掩盖自身产生的鞭毛蛋白,从而逃避植物防御。



微生物可以作为内生菌进入健康的植物并赋予有益的功能。共生微生物进入植物涉及穿透植物防御。大多数克服植物防御的机制都集中在适应的病原体上,而有益内生菌逃避植物防御以实现和谐共生的机制尚不清楚。在这里,我们发现了一种内生细菌枯草芽孢杆菌 BSn5 通过产生羊毛硫抗生素枯草霉素来结合自身产生的鞭毛蛋白来刺激植物防御反应的机制。 Subtilomycin 与鞭毛蛋白结合并影响 flg22 诱导的植物防御,从而促进拟南芥内生菌的定殖。枯草霉素还促进 BSn5 在一种独特的植物魔芋中定植,BSn5 是在魔芋中分离出来的。我们的调查表明,更多独立的枯草霉素/类生产者是从不同的植物中分离出来的。我们的工作揭示了一种用于细菌内生定植的通用策略。
更新日期:2019-10-10
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