Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell-Adhesive,ACS Applied Materials & Interfaces

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Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell-Adhesive
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-10-24 , DOI: 10.1021/acsami.9b16025
David B. Gehlen ₁ , Leticia C. De Lencastre Novaes ₁ , Wei Long ₂ , Anna Joelle Ruff ₂ , Felix Jakob ₂ , Tamás Haraszti ₁ , Yashoda Chandorkar ₁ , Liangliang Yang ₃ , Patrick van Rijn ₃ , Ulrich Schwaneberg ₂ , Laura De Laporte _{1,

4}

Affiliation

Polydimethylsiloxane (PDMS) is a synthetic material with excellent properties for biomedical applications because of its easy fabrication method, high flexibility, permeability to oxygen, transparency, and potential to produce high-resolution structures in the case of lithography. However, PDMS needs to be modified to support homogeneous cell attachments and spreading. Even though many physical and chemical methods, like plasma treatment or extracellular matrix coatings, have been developed over the last decades to increase cell–surface interactions, these methods are still very time-consuming, often not efficient enough, complex, and can require several treatment steps. To overcome these issues, we present a novel, robust, and fast one-step PDMS coating method using engineered anchor peptides fused to the cell-adhesive peptide sequence (glycine-arginine-glycine-aspartate-serine, GRGDS). The anchor peptide attaches to the PDMS surface predominantly by hydrophobic interactions by simply dipping PDMS in a solution containing the anchor peptide, presenting the GRGDS sequence on the surface available for cell adhesion. The binding performance and kinetics of the anchor peptide to PDMS are characterized, and the coatings are optimized for efficient cell attachment of fibroblasts and endothelial cells. Additionally, the applicability is proven using PDMS-based directional nanotopographic gradients, showing a lower threshold of 5 μm wrinkles for fibroblast alignment.

中文翻译：

快速而坚固的涂覆方法使聚二甲基硅氧烷表面具有细胞粘合性

聚二甲基硅氧烷（PDMS）是一种合成材料，在生物医学领域具有优异的性能，这是因为其易于制造的方法，高柔韧性，透氧性，透明性以及在光刻情况下具有产生高分辨率结构的潜力。但是，PDMS需要修改以支持均匀的细胞附着和扩散。尽管在过去的几十年中已经开发出许多物理和化学方法，例如等离子体处理或细胞外基质涂层，以增加细胞表面的相互作用，但这些方法仍然非常耗时，通常效率不高，复杂，并且可能需要数种方法。治疗步骤。为了克服这些问题，我们提出了一种新颖，强大，和快速的一步法PDMS包被方法，使用融合到细胞粘附肽序列上的工程化锚定肽（甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸，GRGDS）。通过简单地将PDMS浸入含有锚肽的溶液中，锚肽主要通过疏水相互作用而附着于PDMS表面，从而在表面上呈现出可用于细胞粘附的GRGDS序列。表征了锚定肽与PDMS的结合性能和动力学，并且对涂层进行了优化以使成纤维细胞和内皮细胞有效附着。此外，使用基于PDMS的定向纳米形貌梯度证明了其适用性，对于成纤维细胞排列，皱纹的阈值较低，仅为5μm。通过简单地将PDMS浸入含有锚肽的溶液中，锚肽主要通过疏水相互作用而附着于PDMS表面，从而在表面上呈现出可用于细胞粘附的GRGDS序列。表征了锚定肽与PDMS的结合性能和动力学，并对涂层进行了优化，以有效地连接成纤维细胞和内皮细胞。此外，使用基于PDMS的定向纳米形貌梯度证明了其适用性，对于成纤维细胞排列，皱纹的阈值较低，仅为5μm。通过简单地将PDMS浸入含有锚肽的溶液中，锚肽主要通过疏水相互作用而附着于PDMS表面，从而在表面上呈现出可用于细胞粘附的GRGDS序列。表征了锚定肽与PDMS的结合性能和动力学，并对涂层进行了优化，以有效地连接成纤维细胞和内皮细胞。此外，使用基于PDMS的定向纳米形貌梯度证明了其适用性，对于成纤维细胞排列，皱纹的阈值较低，仅为5μm。优化了涂层，使成纤维细胞和内皮细胞有效附着在细胞上。此外，使用基于PDMS的定向纳米形貌梯度证明了其适用性，显示出用于成纤维细胞排列的5μm皱纹的较低阈值。优化了涂层，使成纤维细胞和内皮细胞有效附着在细胞上。此外，使用基于PDMS的定向纳米形貌梯度证明了其适用性，对于成纤维细胞排列，皱纹的阈值较低，仅为5μm。

更新日期：2019-10-25

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