减数分裂的交汇点，其特征在于玉米单倍型特异的染色体绘画。,Nature Communications

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减数分裂的交汇点，其特征在于玉米单倍型特异的染色体绘画。
Nature Communications ( IF 14.7 ) Pub Date : 2019-10-10 , DOI: 10.1038/s41467-019-12646-z
Lívia do Vale Martins _{1,

2} , Fan Yu _{1,

2,

3} , Hainan Zhao _{1,

2} , Tesia Dennison ₄ , Nick Lauter _{4,

5} , Haiyan Wang _{1,

2} , Zuhu Deng ₃ , Addie Thompson _{6,

7} , Kassandra Semrau _{8,

9} , Jean-Marie Rouillard _{8,

10} , James A Birchler ₁₁ , Jiming Jiang _{1,

2,

7}

Affiliation

Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA.
National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
Genetics and Genomics Graduate Program, Iowa State University, Ames, IA, 50011, USA.
USDA-ARS Corn Insects and Crop Genetics Research Unit, Iowa State University, Ames, IA, 50011, USA.
Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA.
Michigan State University AgBioResearch, East Lansing, MI, 48824, USA.
Arbor Biosciences, Ann Arbor, MI, 48103, USA.
Department of Natural Sciences, University of Michigan-Dearborn, Dearborn, MI, 48128, USA.
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Division of Biological Sciences, University of Missouri, Columbia, MO, 65211, USA.

减数分裂交换（COs）在减数分裂过程中产生遗传变异和维持同源染色体的忠实分离方面发挥着关键作用。我们开发了一种单倍型特异性荧光原位杂交（FISH）技术，该技术可直接在中期染色体上可视化COs。分别合成了玉米自交系B73和Mo17的10号染色体特异的寡核苷酸（寡核苷酸），并将其标记为FISH探针。B73 x Mo17 F1杂种和F2后代中的亲本和重组10号染色体可以通过单倍型特异性FISH明确鉴定。对58株F2植物的分析显示，在10号染色体的整个近端中都缺少CO，但是，我们从一个确定的B73 x Mo17种群的重组自交系中检测到了非常靠近着丝粒的区域中的CO。

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更新日期：2019-10-10

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