A dynamic 3D DNA nanostructure based on silicon-supported lipid bilayers: a highly efficient DNA nanomachine for rapid and sensitive sensing†,Chemical Communications

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A dynamic 3D DNA nanostructure based on silicon-supported lipid bilayers: a highly efficient DNA nanomachine for rapid and sensitive sensing†
Chemical Communications ( IF 4.3 ) Pub Date : 2019-10-09 , DOI: 10.1039/c9cc07071k
Zhi-Bin Wen _{1,

2,

3,

4,

5} , Xin Peng _{1,

2,

3,

4,

5} , Ze-Zhou Yang _{1,

2,

3,

4,

5} , Ying Zhuo _{1,

2,

3,

4,

5} , Ya-Qin Chai _{1,

2,

3,

4,

5} , Wen-Bin Liang _{1,

2,

3,

4,

5} , Ruo Yuan _{1,

2,

3,

4,

5}

Affiliation

Herein, by anchoring cholesterol-labelled DNA probes to silicon-supported lipid bilayers via cholesterol–lipid interaction, a dynamic three-dimensional (3D) DNA nanostructure could be facilely assembled, which is applied as a microRNA (miRNA)-induced self-powered 3D DNA nanomachine with high movement efficiency. Once the self-powered 3D DNA nanomachine is triggered by target miRNA, it achieves autonomous operation without external addition of fuel DNA strands or protein enzymes. Impressively, the biocompatible lipid bilayers not only preserve the biological character of the DNA probes, but also improve the movement efficiency of the DNA nanomachine, which directly solves the key challenge of the steric barrier effect of traditional rigid surfaces (Au or silicon) for DNA probe diffusion. As a proof of concept, our proposed DNA nanomachine is successfully applied in rapid and sensitive detection of miRNAs, which gives a new idea for the construction of highly efficient DNA nanomachines for biosensing and clinic diagnosis.

中文翻译：

基于硅支持的脂质双层的动态3D DNA纳米结构：用于快速灵敏传感的高效DNA纳米机器†

在这里，通过将胆固醇标记的DNA探针通过胆固醇-脂质相互作用，可以轻松组装动态三维（3D）DNA纳米结构，该技术可作为microRNA（miRNA）诱导的自供电3D DNA纳米机器，具有很高的运动效率。一旦目标miRNA触发了自供电的3D DNA纳米机，它就可以实现自主运行，而无需外部添加燃料DNA链或蛋白质酶。令人印象深刻的是，具有生物相容性的脂质双层不仅保留了DNA探针的生物学特性，而且还提高了DNA纳米机器的移动效率，这直接解决了传统刚性表面（Au或硅）对DNA的空间屏障作用的关键挑战探针扩散。作为概念的证明，我们提出的DNA纳米机已成功应用于miRNA的快速灵敏检测，

更新日期：2019-11-05

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