cylindrosperpsoppsin对斑马鱼（Danio rerio）胚胎发育的不利影响。,Chemosphere

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cylindrosperpsoppsin对斑马鱼（Danio rerio）胚胎发育的不利影响。
Chemosphere ( IF 8.1 ) Pub Date : 2019-10-09 , DOI: 10.1016/j.chemosphere.2019.125060
Linping Wang ₁ , Qilong Wang ₂ , Guosheng Xiao ₂ , Guoliang Chen ₁ , Lin Han ₂ , Tingzhang Hu ₁

Affiliation

淡水体的富营养化增加了有毒蓝藻水华的发生。蓝藻毒素cylindrospermopsin（CYN）由于在水体中的存在越来越多而引起了极大的兴趣。然而，人们对CYN对斑马鱼发育的毒性作用知之甚少，尤其是其毒理学机理尚不清楚。在这项研究中，我们检查了CYN对斑马鱼胚胎发育的不利影响。CYN（2-2000 nM）暴露以浓度依赖的方式降低了胚胎的存活率，孵化率，体长和眼睛大小，并导致胚胎形态异常，包括心包水肿，脊柱弯曲，尾巴畸形，未充气的游泳膀胱，心脏和血管缺陷。浓度为20 nM或更高的CYN可以显着增加ROS水平并促进斑马鱼胚胎中的细胞凋亡。为了初步阐明由CYN引起的斑马鱼发育毒性的潜在机制，我们检查了氧化应激和凋亡相关基因的表达。CYN可以促进氧化应激相关基因（SOD1，CAT和GPx1）的表达，并诱导凋亡相关基因（p53，Bax和Bcl-2）的转录水平发生变化。两者合计，CYN诱导对斑马鱼胚胎发育的不利影响，这可能与氧化应激和细胞凋亡有关。这些结果将增进我们对由气候变化和富营养化引起的CYN毒性，环境问题和健康危害的理解。为了初步阐明由CYN引起的斑马鱼发育毒性的潜在机制，我们检查了氧化应激和凋亡相关基因的表达。CYN可以促进氧化应激相关基因（SOD1，CAT和GPx1）的表达，并诱导凋亡相关基因（p53，Bax和Bcl-2）的转录水平发生变化。两者合计，CYN诱导对斑马鱼胚胎发育的不利影响，这可能与氧化应激和细胞凋亡有关。这些结果将增进我们对由气候变化和富营养化引起的CYN毒性，环境问题和健康危害的理解。为了初步阐明由CYN引起的斑马鱼发育毒性的潜在机制，我们检查了氧化应激和凋亡相关基因的表达。CYN可以促进氧化应激相关基因（SOD1，CAT和GPx1）的表达，并诱导凋亡相关基因（p53，Bax和Bcl-2）的转录水平发生变化。两者合计，CYN诱导对斑马鱼胚胎发育的不利影响，这可能与氧化应激和细胞凋亡有关。这些结果将增进我们对由气候变化和富营养化引起的CYN毒性，环境问题和健康危害的理解。CAT和GPx1）并诱导凋亡相关基因（p53，Bax和Bcl-2）的转录水平发生变化。两者合计，CYN诱导对斑马鱼胚胎发育的不利影响，这可能与氧化应激和细胞凋亡有关。这些结果将增进我们对由气候变化和富营养化引起的CYN毒性，环境问题和健康危害的理解。CAT和GPx1）并诱导凋亡相关基因（p53，Bax和Bcl-2）的转录水平发生变化。两者合计，CYN诱导对斑马鱼胚胎发育的不利影响，这可能与氧化应激和细胞凋亡有关。这些结果将增进我们对由气候变化和富营养化引起的CYN毒性，环境问题和健康危害的理解。

"点击查看英文标题和摘要"

Adverse effect of cylindrospermopsin on embryonic development in zebrafish (Danio rerio).

Eutrophication of freshwater bodies increases the occurrence of toxic cyanobacterial blooms. The cyanobacterial toxin cylindrospermopsin (CYN) is receiving great interest due to its increasing presence in waterbodies. However, the toxic effects of CYN on zebrafish development are poorly understood, especially the toxicological mechanism, which is still unclear. In this study, we examined the adverse effects of CYN on embryonic development in zebrafish. CYN (2-2000 nM) exposure decreased embryos survival rate, hatching rate, body length and eye size in a concentration-dependent manner and caused abnormalities in embryo morphology, including pericardial edema, spinal curvature, tail deformity, uninflated swim bladder, cardiac and vascular defects. CYN at concentrations of 20 nM or higher significantly increased ROS level and promoted cell apoptosis in zebrafish embryos. To preliminarily elucidate the potential mechanism of zebrafish developmental toxicity caused by CYN, we examined the expression of oxidative stress- and apoptotic-related genes. CYN could promote the expression of oxidative stress-related genes (SOD1, CAT and GPx1) and induce changes in transcriptional levels of apoptotic-related genes (p53, Bax and Bcl-2). Taken together, CYN induced adverse effects on zebrafish embryos development, which may associate with oxidative stress and apoptosis. These outcomes will advance our understanding of CYN toxicity, environmental problems and health hazards caused by climate changes and eutrophication.

更新日期：2019-10-10

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