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Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-11-25 , DOI: 10.1021/acs.jafc.9b02690 Holly Stolterfoht 1 , Claudia Rinnofner 1, 2 , Margit Winkler 1, 3 , Harald Pichler 1, 3
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-11-25 , DOI: 10.1021/acs.jafc.9b02690 Holly Stolterfoht 1 , Claudia Rinnofner 1, 2 , Margit Winkler 1, 3 , Harald Pichler 1, 3
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Green leaf volatiles (GLVs) are mainly C6- and in rare cases also C9-aldehydes, -alcohols, and -esters, which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focuses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids, such as linoleic and α-linolenic acids. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications, plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability, and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.
中文翻译：
重组脂氧合酶和氢过氧化物裂解酶，用于合成绿叶挥发物。
绿叶挥发物（GLV）主要是C6-，在极少数情况下，也是植物响应生物或非生物胁迫而释放的C9-醛，-醇和-酯。这些化合物以其特有的气味让人联想到刚割过的草而得名。这篇评论集中在GLV和负责其形成的两个主要途径酶：脂氧合酶（LOXs）和脂肪酸氢过氧化物裂解酶（HPLs）。LOX催化不饱和脂肪酸（例如亚油酸和α-亚麻酸）的过氧化。氢过氧脂肪酸被HPL进一步转化为醛和含氧酸。在许多工业应用中，植物提取物已被用作LOX和HPL来源。但是，这些过程受到低酶浓度，稳定性和特异性的限制。或者，重组酶可用作GLV合成的生物催化剂。由微生物宿主有效表达的特征化酶的数量不断增加，将促进GLV生产的创新性生物催化工艺的发展。
更新日期：2019-11-28
中文翻译：
重组脂氧合酶和氢过氧化物裂解酶，用于合成绿叶挥发物。
绿叶挥发物（GLV）主要是C6-，在极少数情况下，也是植物响应生物或非生物胁迫而释放的C9-醛，-醇和-酯。这些化合物以其特有的气味让人联想到刚割过的草而得名。这篇评论集中在GLV和负责其形成的两个主要途径酶：脂氧合酶（LOXs）和脂肪酸氢过氧化物裂解酶（HPLs）。LOX催化不饱和脂肪酸（例如亚油酸和α-亚麻酸）的过氧化。氢过氧脂肪酸被HPL进一步转化为醛和含氧酸。在许多工业应用中，植物提取物已被用作LOX和HPL来源。但是，这些过程受到低酶浓度，稳定性和特异性的限制。或者，重组酶可用作GLV合成的生物催化剂。由微生物宿主有效表达的特征化酶的数量不断增加，将促进GLV生产的创新性生物催化工艺的发展。
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