Plasmonic Gold Nanorattle Impregnated Chitosan Nanocarrier for Stimulus Responsive Theranostics,ACS Applied Bio Materials

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Plasmonic Gold Nanorattle Impregnated Chitosan Nanocarrier for Stimulus Responsive Theranostics
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-10-23 , DOI: 10.1021/acsabm.9b00568
Ankita Sarkar ₁ , Shounak Roy ₁ , Pallab Sanpui ₂ , Amit Jaiswal ₁

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Herein, a stimulus-responsive theranostic nanosystem comprising gold nanorattles (AuNRTs), having a solid octahedron core and thin porous cubic shell, encapsulated within chitosan nanocarriers (CS-AuNRT) has been reported. Due to the plasmonic AuNRTs, CS-AuNRT demonstrated unique features of near infrared (NIR) absorbance and accessible intrinsic electromagnetic “hot spots” arising due to coupling of inner solid core and outer porous shell. These properties enabled CS-AuNRTs to be used for NIR-responsive drug delivery, photothermal therapy, and surface enhanced Raman scattering (SERS) based bioimaging. Following loading of chemotherapeutic drug doxorubicin (DOX) within AuNRTs along with a phase changing material (PCM), application of NIR irradiation resulted in photothermal melting of the PCM and simultaneous payload release in the surrounding medium. Although being nontoxic themselves, CS-AuNRTs with or without loaded DOX could mount significant cell death in breast cancer cell line (MCF-7) in the presence of NIR light as external stimulus. The oxidative stress generated by DOX-loaded and empty CS-AuNRTs upon NIR irradiation were confirmed by flow-cytometric determination of intracellular reactive oxygen species (ROS). Further, the ROS-led induction of apoptosis in treated MCF-7 cells was established from characteristic nuclear fragmentation, morphological changes and membrane blebbing as observed through confocal fluorescence and scanning electron microscopy. Thus, with NIR responsive chemo-photothermal therapy and SERS based bioimaging, the present nanocarrier system holds potential for cancer theranostics.

中文翻译：

Plasmonic Gold Nanorattle 浸渍壳聚糖纳米载体用于刺激响应治疗

本文报道了一种刺激响应的治疗诊断纳米系统，包括金纳米晶 (AuNRT)，具有实心八面体核心和薄多孔立方壳，封装在壳聚糖纳米载体 (CS-AuNRT) 中。由于等离子体AuNRT，CS-AuNRT表现出近红外（NIR）吸收的独特特征和由于内部实心核和外部多孔壳的耦合而产生的可接近的固有电磁“热点”。这些特性使 CS-AuNRT 能够用于近红外响应药物递送、光热疗法和基于表面增强拉曼散射 (SERS) 的生物成像。在 AuNRT 中加载化学治疗药物阿霉素 (DOX) 以及相变材料 (PCM) 后，近红外辐射的应用导致 PCM 的光热熔化并同时在周围介质中释放有效载荷。尽管本身无毒，但在 NIR 光作为外部刺激存在的情况下，无论是否加载 DOX，CS-AuNRT 都可以在乳腺癌细胞系 (MCF-7) 中引起显着的细胞死亡。通过细胞内活性氧 (ROS) 的流式细胞仪测定证实了在 NIR 照射下负载 DOX 和空 CS-AuNRT 产生的氧化应激。此外，通过共聚焦荧光和扫描电子显微镜观察到的特征性核碎裂、形态变化和膜起泡，确定了经处理的 MCF-7 细胞中 ROS 诱导的细胞凋亡。因此，通过近红外响应化学光热疗法和基于 SERS 的生物成像，

更新日期：2019-10-24

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