Nanostructured Substrates for Detection and Characterization of Circulating Rare Cells: From Materials Research to Clinical Applications.,Advanced Materials

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Nanostructured Substrates for Detection and Characterization of Circulating Rare Cells: From Materials Research to Clinical Applications.
Advanced Materials ( IF 27.4 ) Pub Date : 2019-09-30 , DOI: 10.1002/adma.201903663
Jiantong Dong _{1,

2} , Jie-Fu Chen ₃ , Matthew Smalley ₁ , Meiping Zhao ₂ , Zunfu Ke ₄ , Yazhen Zhu ₁ , Hsian-Rong Tseng ₁

Affiliation

Circulating rare cells in the blood are of great significance for both materials research and clinical applications. For example, circulating tumor cells (CTCs) have been demonstrated as useful biomarkers for "liquid biopsy" of the tumor. Circulating fetal nucleated cells (CFNCs) have shown potential in noninvasive prenatal diagnostics. However, it is technically challenging to detect and isolate circulating rare cells due to their extremely low abundance compared to hematologic cells. Nanostructured substrates offer a unique solution to address these challenges by providing local topographic interactions to strengthen cell adhesion and large surface areas for grafting capture agents, resulting in improved cell capture efficiency, purity, sensitivity, and reproducibility. In addition, rare-cell retrieval strategies, including stimulus-responsiveness and additive reagent-triggered release on different nanostructured substrates, allow for on-demand retrieval of the captured CTCs/CFNCs with high cell viability and molecular integrity. Several nanostructured substrate-enabled CTC/CFNC assays are observed maturing from enumeration and subclassification to molecular analyses. These can one day become powerful tools in disease diagnosis, prognostic prediction, and dynamic monitoring of therapeutic response-paving the way for personalized medical care.

中文翻译：

用于循环稀有细胞检测和表征的纳米结构基底：从材料研究到临床应用。

血液中循环的稀有细胞对于材料研究和临床应用都具有重要意义。例如，循环肿瘤细胞（CTC）已被证明是肿瘤“液体活检”的有用生物标志物。循环胎儿有核细胞（CFNC）在无创产前诊断中显示出潜力。然而，由于与血液细胞相比，循环稀有细胞的丰度极低，因此检测和分离循环稀有细胞在技术上具有挑战性。纳米结构基底提供了一种独特的解决方案来应对这些挑战，通过提供局部形貌相互作用来增强细胞粘附，并为接枝捕获剂提供大的表面积，从而提高细胞捕获效率、纯度、灵敏度和重现性。此外，稀有细胞修复策略，包括不同纳米结构基质上的刺激响应性和添加剂触发释放，允许按需修复捕获的具有高细胞活力和分子完整性的 CTC/CFNC。观察到几种支持纳米结构基质的 CTC/CFNC 检测从计数和子分类到分子分析的成熟。这些有一天可能成为疾病诊断、预后预测和治疗反应动态监测的强大工具，为个性化医疗护理铺平道路。

更新日期：2020-01-07

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