Ultrahighly Efficient and Stable Fluorescent Gold Nanoclusters Coated with Screened Peptides of Unique Sequences for Effective Protein and Serum Discrimination,Analytical Chemistry

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Ultrahighly Efficient and Stable Fluorescent Gold Nanoclusters Coated with Screened Peptides of Unique Sequences for Effective Protein and Serum Discrimination
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-10-10 , DOI: 10.1021/acs.analchem.9b03463
Shenghao Xu ₁ , Wentao Li ₁ , Xuan Zhao ₁ , Tong Wu ₁ , Yanyun Cui ₂ , Xinyue Fan ₃ , Wei Wang ₁ , Xiliang Luo ₁

Affiliation

Gold nanoclusters (Au NCs) coated with various peptides have been widely used as fluorescent probes, and nowadays the most commonly used are cysteine (C) and tyrosine (Y) based ones. Herein, we report the preparation and clinical application of highly efficient and stable fluorescent Au NCs protected by screened peptides with a specific amino acid sequence Cys-Met-Met-Met-Met-Met (CMMMMM). Compared with traditional C, Y based peptide (CYYYYY) protected Au NCs, the fluorescence intensity of the CMMMMM-Au NCs increased by 230%, and the photobleaching resistance or stability of the CMMMMM-Au NCs increased by about 300% (after continuous ultraviolet irradiation for 60 min, the fluorescence of the CMMMMM-Au NCs remained more than 90% of their initial intensity, while the CYYYYY-Au NCs remained less than 30%). Assaying arrays based on CMMMMM protected Au NCs with different positive or negative charges as sensing receptors were developed through regulating different pH values, and multivariate analysis on the patterns obtained by these arrays allowed effective identification of not only ten proteins separately but also complex protein mixtures with subtly diverse compositions. The docking simulation and isothermal titration confirmed that target proteins interacted with CMMMMM-Au NCs mainly through electrostatic interactions and partly hydrophobic interactions, which affected the binding energy and fluorescence lifetime of CMMMMM-Au NCs, resulting in the unique fingerprint-like recognition patterns. Furthermore, serums from breast cancer, severe osteoarthritis, and rectal cancer patients can be effectively identified with healthy people using this CMMMMM-Au NCs based sensor array.

中文翻译：

超高效和稳定的荧光金纳米簇，其包覆有有效蛋白质和血清区分的独特序列的筛选肽

涂有各种肽的金纳米簇（Au NCs）已被广泛用作荧光探针，如今最常用的是基于半胱氨酸（C）和酪氨酸（Y）的探针。在本文中，我们报告了由具有特定氨基酸序列Cys-Met-Met-Met-Met-Met（CMMMMM）的筛选肽保护的高效，稳定的荧光Au Au的制备和临床应用。与传统的C，Y基肽（CYYYYY）保护的Au NCs相比，CMMMMM-Au NCs的荧光强度提高了230％，并且CMMMMM-Au NCs的耐光漂白性或稳定性提高了约300％（连续紫外线照射后）辐照60分钟后，CMMMMM-Au NCs的荧光保持其初始强度的90％以上，而CYYYYY-Au NCs的荧光保持小于30％）。通过调节不同的pH值，开发了基于CMMMMM保护的具有不同正电荷或负电荷作为传感受体的Au NCs的检测阵列，通过对这些阵列获得的模式进行多变量分析，不仅可以有效地鉴定出十种蛋白质，而且还可以有效地鉴定复杂的蛋白质混合物。微妙的组成。对接模拟和等温滴定证实目标蛋白主要通过静电相互作用和部分疏水相互作用与CMMMMM-Au NCs相互作用，这影响了CMMMMM-Au NCs的结合能和荧光寿命，从而产生了独特的指纹样识别模式。此外，来自乳腺癌，严重骨关节炎，

更新日期：2019-10-10

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