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Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-09-24 , DOI: 10.1038/s41598-019-50264-3
Congli Wang 1 , Qiang Fu 1 , Xiong Dun 1 , Wolfgang Heidrich 1
Affiliation  

Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.

中文翻译:

使用快照白光波前传感的定量相和强度显微镜。

相成像技术是显微镜中快速检查薄透明标本的宝贵工具。现有方法仅限于仅产生定性相信息的简单且廉价的方法(例如,相差显微镜,DIC),或复杂得多且昂贵的定量方法。在这里,我们演示了一种低成本,易于实施的显微镜设置,用于使用准直白光照明对相位和明场幅度进行定量成像。
更新日期:2019-09-25
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