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Red–Near-Infrared Fluorescent Probe for Time-Resolved in Vivo Alkaline Phosphatase Detection with the Assistance of a Photoresponsive Nanocontainer
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-10-03 , DOI: 10.1021/acs.analchem.9b03497 Xu Jie 1 , Mei Wu 1 , Haimei Yang 1 , Weili Wei 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-10-03 , DOI: 10.1021/acs.analchem.9b03497 Xu Jie 1 , Mei Wu 1 , Haimei Yang 1 , Weili Wei 1
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The monitoring of alkaline phosphatase (ALP) activity in different tissues is significant for disease diagnosis and therapy. However, the time-resolved in vivo sensing of ALP activity remained unresolved. Herein, a novel red–near-infrared fluorescent ALP probe (Cl2–BDCM-ALP) based on a dichloro-substituted dicyanomethylene-4H-chromene derivative was designed and synthesized with high fluorescence efficiency and stability under biological pH range. By using Cl2–BDCM-ALP, ALP activity under an acidic microenvironment such as a tumor site can be sensitively imaged, which cannot be achieved by some previously reported ALP probes. By further loading the Cl2–BDCM-ALP into a near-infrared (NIR) light-responsive nanocontainer, time-resolved long-term imaging of ALP activity was facilely achieved with noninvasive NIR light remote control. Time-resolved variation of ALP activity of the drug-induced acute liver injury mice was successfully monitored in vivo for the first time. This strategy holds great promise in the in situ ALP detection under a broad pH range with temporal resolution.
中文翻译：
红-近红外荧光探针可在光敏纳米容器的协助下在体内碱性磷酸酶检测中进行时间分辨
监测不同组织中碱性磷酸酶（ALP）的活性对于疾病的诊断和治疗具有重要意义。然而，时间分辨的体内对ALP活性的感测仍未解决。在此，设计并合成了一种基于二氯取代的二氰基亚甲基-4 H-亚甲基苯衍生物的新型红-近红外荧光ALP探针（Cl 2 -BDCM-ALP），并在生物pH范围内具有较高的荧光效率和稳定性。通过使用Cl 2 -BDCM-ALP，可以对酸性微环境（例如肿瘤部位）下的ALP活性进行敏感成像，这是某些先前报道的ALP探针无法实现的。通过进一步加载Cl 2–将BDCM-ALP植入近红外（NIR）光响应纳米容器中，可通过无创NIR光线遥控器轻松实现ALP活性的时间分辨长期成像。首次在体内成功监测了药物诱导的急性肝损伤小鼠的ALP活性随时间变化的变化。该策略在宽广的pH范围内以时间分辨率在原位ALP检测中具有广阔的前景。
更新日期：2019-10-04
中文翻译：
红-近红外荧光探针可在光敏纳米容器的协助下在体内碱性磷酸酶检测中进行时间分辨
监测不同组织中碱性磷酸酶（ALP）的活性对于疾病的诊断和治疗具有重要意义。然而，时间分辨的体内对ALP活性的感测仍未解决。在此，设计并合成了一种基于二氯取代的二氰基亚甲基-4 H-亚甲基苯衍生物的新型红-近红外荧光ALP探针（Cl 2 -BDCM-ALP），并在生物pH范围内具有较高的荧光效率和稳定性。通过使用Cl 2 -BDCM-ALP，可以对酸性微环境（例如肿瘤部位）下的ALP活性进行敏感成像，这是某些先前报道的ALP探针无法实现的。通过进一步加载Cl 2–将BDCM-ALP植入近红外（NIR）光响应纳米容器中，可通过无创NIR光线遥控器轻松实现ALP活性的时间分辨长期成像。首次在体内成功监测了药物诱导的急性肝损伤小鼠的ALP活性随时间变化的变化。该策略在宽广的pH范围内以时间分辨率在原位ALP检测中具有广阔的前景。
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