Fast and compact DNA logic circuits based on single-stranded gates using strand-displacing polymerase.,Nature Nanotechnology

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Fast and compact DNA logic circuits based on single-stranded gates using strand-displacing polymerase.
Nature Nanotechnology ( IF 38.1 ) Pub Date : 2019-09-23 , DOI: 10.1038/s41565-019-0544-5
Tianqi Song ₁ , Abeer Eshra ₂ , Shalin Shah ₃ , Hieu Bui ₄ , Daniel Fu ₁ , Ming Yang ₁ , Reem Mokhtar ₁ , John Reif _{1,

3}

Affiliation

DNA is a reliable biomolecule with which to build molecular computation systems. In particular, DNA logic circuits (diffusion-based) have shown good performance regarding scalability and correctness of computation. However, previous architectures of DNA logic circuits have two limitations. First, the speed of computation is slow, often requiring hours to compute a simple function. Second, the circuits are of high complexity regarding the number of DNA strands. Here, we introduce an architecture of DNA logic circuits based on single-stranded logic gates using strand-displacing DNA polymerase. The logic gates consist of only single DNA strands, which largely reduces leakage reactions and signal restoration steps such that the circuits are improved in regard to both speed of computation and the number of DNA strands needed. Large-scale logic circuits can be constructed from the gates by simple cascading strategies. In particular, we have demonstrated a fast and compact logic circuit that computes the square-root function of four-bit input numbers.

中文翻译：

使用链置换聚合酶基于单链门的快速紧凑的DNA逻辑电路。

DNA是构建分子计算系统的可靠生物分子。特别是，DNA逻辑电路（基于扩散）在扩展性和计算正确性方面表现出良好的性能。但是，DNA逻辑电路的先前体系结构有两个局限性。首先，计算速度很慢，通常需要数小时才能计算出简单的函数。其次，关于DNA链的数量，电路具有很高的复杂性。在这里，我们介绍一种基于单链逻辑门的DNA逻辑电路架构，该单链逻辑门使用链置换DNA聚合酶。逻辑门仅由一条DNA链组成，这大大减少了泄漏反应和信号恢复步骤，从而在计算速度和所需DNA链数方面都改善了电路。可以通过简单的级联策略从门构建大规模逻辑电路。特别是，我们展示了一种快速而紧凑的逻辑电路，该电路可计算四位输入数字的平方根函数。

更新日期：2019-09-23

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