Microfluidic Single-Cell Omics Analysis.,Small

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Microfluidic Single-Cell Omics Analysis.
Small ( IF 13.0 ) Pub Date : 2019-09-23 , DOI: 10.1002/smll.201903905
Xing Xu ₁ , Junxia Wang ₁ , Lingling Wu ₂ , Jingjing Guo ₁ , Yanling Song ₂ , Tian Tian ₁ , Wei Wang ₂ , Zhi Zhu ₁ , Chaoyong Yang _{1,

2}

Affiliation

The commonly existing cellular heterogeneity plays a critical role in biological processes such as embryonic development, cell differentiation, and disease progress. Single-cell omics-based heterogeneous studies have great significance for identifying different cell populations, discovering new cell types, revealing informative cell features, and uncovering significant interrelationships between cells. Recently, microfluidics has evolved to be a powerful technology for single-cell omics analysis due to its merits of throughput, sensitivity, and accuracy. Herein, the recent advances of microfluidic single-cell omics analysis, including different microfluidic platform designs, lysis strategies, and omics analysis techniques, are reviewed. Representative applications of microfluidic single-cell omics analysis in complex biological studies are then summarized. Finally, a few perspectives on the future challenges and development trends of microfluidic-assisted single-cell omics analysis are discussed.

中文翻译：

微流体单细胞组学分析。

普遍存在的细胞异质性在诸如胚胎发育，细胞分化和疾病进展等生物学过程中起着至关重要的作用。基于单细胞组学的异质性研究对于鉴定不同细胞群体，发现新细胞类型，揭示信息性细胞特征以及揭示细胞之间重要的相互关系具有重要意义。最近，由于微流体技术的通量，灵敏度和准确性的优点，它已发展成为一种用于单细胞组学分析的强大技术。本文中，综述了微流体单细胞组学分析的最新进展，包括不同的微流体平台设计，裂解策略和组学分析技术。然后总结了微流体单细胞组学分析在复杂生物学研究中的代表性应用。最后，讨论了微流体辅助单细胞组学分析的未来挑战和发展趋势的一些观点。

更新日期：2020-03-05

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