卵磷脂添加物在含有姜黄素的壳聚糖和硫酸软骨素纳米颗粒的性质和细胞毒性活性中的作用。,Carbohydrate Polymers

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卵磷脂添加物在含有姜黄素的壳聚糖和硫酸软骨素纳米颗粒的性质和细胞毒性活性中的作用。
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2019-09-21 , DOI: 10.1016/j.carbpol.2019.115351
Katiúscia Vieira Jardim ₁ , Joseilma Luciana Neves Siqueira ₂ , Sônia Nair Báo ₂ , Marcelo Henrique Sousa ₁ , Alexandre Luis Parize ₃

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表面活性剂已被用作通过这些纳米颗粒改善聚合物纳米颗粒（NP）的性能并提高疏水性药物释放速率的工具。在这种情况下，这项研究评估了卵磷脂在姜黄素（Curc）释放中对壳聚糖（CHI）和硫酸软骨素（CS）纳米颗粒特性的影响。CHI / CS NPs和CHI / CS /卵磷脂NPs是通过离子凝胶法制备的，既作为标准品，又含有姜黄素。同时进行电导和电位滴定以优化聚合物之间的相互作用。获得了水动力直径约为130 nm，ζ电位为+60 mV的NP，并通过HRTEM对其进行了表征。还通过BET方法，DLS，FTIR，XPS对它们的孔径和表面积进行了分析，和荧光光谱技术来评估形态和表面特性，聚合物之间的稳定性和相互作用，并量化药物的载量。NP的最终特性直接受到卵磷脂添加的影响，表现出姜黄素的包封效率提高（每毫克CHI / CS /卵磷脂/ Curc NPs含有131.8μg姜黄素）。姜黄素的释放通过两个阶段逐渐发生：扩散控制的溶解和姜黄素的释放（通过聚合物的溶解控制）。但是，与CHI / CS / Curc NPs相比，CHI / CS /卵磷脂/ Curc NPs在pH 7.4的缓冲溶液中释放姜黄素的速度更快。用MTT法测定姜黄素的体外细胞毒性活性，观察到在CHI / CS / Curc和CHI / CS /卵磷脂/ Curc NP中纳米包裹的游离姜黄素和姜黄素会降低MCF-7细胞在72小时内的存活率（分别降低28.4％，36.0％和30.7％，P <0.0001）。这些结果表明，CHI / CS /卵磷脂NP具有更合适的姜黄素封装特性。

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The role of the lecithin addition in the properties and cytotoxic activity of chitosan and chondroitin sulfate nanoparticles containing curcumin.

Surfactants have been used as a tool to improve the properties of polymeric nanoparticles (NPs) and to increase the rate of hydrophobic drug release by means of these nanoparticles. In this context, this study evaluated the effect of lecithin on the characteristics of chitosan (CHI) and chondroitin sulfate (CS) nanoparticles, when applied in curcumin (Curc) release. CHI/CS NPs and CHI/CS/Lecithin NPs were prepared by the ionic gelation method, both as standards and containing curcumin. Simultaneous conductimetric and potentiometric titrations were employed to optimize the interaction between the polymers. NPs with hydrodynamic diameter of ∼130 nm and zeta potential of +60 mV were obtained and characterized by HRTEM; their pore size and surface area were also analyzed by BET method, DLS, FTIR, XPS, and fluorescence spectroscopy techniques to assess morphological and surface properties, stability and interaction between polymers and to quantify the loading of drugs. The final characteristics of NPs were directly influenced by lecithin addition, exhibiting enhanced encapsulation efficiency of curcumin (131.8 μg curcumin per mg CHI/CS/Lecithin/Curc NPs). The release of curcumin occurred gradually through a two-stage process: diffusion-controlled dissolution and release of curcumin controlled by dissolution of the polymer. However, the release of curcumin in buffer solution at pH 7.4 was achieved faster in CHI/CS/Lecithin/Curc NPs than in CHI/CS/Curc NPs. in vitro cytotoxic activity evaluation of the curcumin was determined by the MTT assay, observing that free curcumin and curcumin nanoencapsulated in CHI/CS/Curc and CHI/CS/Lecithin/Curc NPs reduced the viability of MCF-7 cells in the 72 h period (by 28.4, 36.0 and 30.7%, P < 0.0001, respectively). These results indicate that CHI/CS/Lecithin NPs have more appropriate characteristics for encapsulation of curcumin.

更新日期：2019-09-22

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