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PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15.
Developmental Cell ( IF 10.7 ) Pub Date : 2019-09-19 , DOI: 10.1016/j.devcel.2019.08.008 Xi Wang 1 , Yanglin Ding 1 , Zhuoyang Li 1 , Yiting Shi 1 , Jinlong Wang 2 , Jian Hua 3 , Zhizhong Gong 1 , Jian-Min Zhou 2 , Shuhua Yang 1
Developmental Cell ( IF 10.7 ) Pub Date : 2019-09-19 , DOI: 10.1016/j.devcel.2019.08.008 Xi Wang 1 , Yanglin Ding 1 , Zhuoyang Li 1 , Yiting Shi 1 , Jinlong Wang 2 , Jian Hua 3 , Zhizhong Gong 1 , Jian-Min Zhou 2 , Shuhua Yang 1
Affiliation
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Cold stress adversely affects plant growth, development, and crop productivity and quality. Plants employ cold acclimation strategy to protect them from cold damage. The transcription-factor-CBF-dependent cold signaling pathway plays a key role in plant cold acclimation. However, how this signaling pathway is dynamically and precisely regulated remains unknown. Here, we report that two U-box type E3 ubiquitin ligases, PUB25 and PUB26, positively regulate freezing tolerance in Arabidopsis thaliana. Both PUB25 and PUB26 poly-ubiquitinate MYB15, a transcriptional repressor of the CBF-dependent cold signaling pathway, leading to MYB15 degradation and thus enhanced CBF expression under cold stress. Furthermore, cold-activated OST1 specifically phosphorylates PUB25 and PUB26 at conserved threonine residues, enhancing their E3 activity and facilitating the cold-induced degradation of MYB15. Our results thus unravel the regulatory role of the OST1-PUB25/26 module in regulating the duration and amplitude of the cold response by controlling the homeostasis of the negative regulator MYB15.
中文翻译：
PUB25和PUB26通过降低冷信号负调节剂MYB15来提高植物的耐寒性。
冷胁迫对植物的生长，发育以及作物的生产力和质量产生不利影响。植物采用冷驯化策略来保护它们免受冷害。转录因子-CBF依赖的冷信号通路在植物冷驯化中起关键作用。然而，如何动态且精确地调节该信号传导途径仍是未知的。在这里，我们报告两个U框类型E3泛素连接酶，PUB25和PUB26，正调控拟南芥的冷冻耐受性。PUB25和PUB26多聚泛素化MYB15是CBF依赖的冷信号通路的转录阻遏物，导致MYB15降解，从而增强了冷胁迫下CBF的表达。此外，冷激活的OST1在保守的苏氨酸残基上特异性磷酸化PUB25和PUB26，增强其E3活性并促进冷诱导的MYB15降解。因此，我们的结果阐明了OST1-PUB25 / 26模块在通过调节负调节剂MYB15的稳态来调节冷反应的持续时间和幅度中的调节作用。
更新日期：2019-09-19
中文翻译：
PUB25和PUB26通过降低冷信号负调节剂MYB15来提高植物的耐寒性。
冷胁迫对植物的生长，发育以及作物的生产力和质量产生不利影响。植物采用冷驯化策略来保护它们免受冷害。转录因子-CBF依赖的冷信号通路在植物冷驯化中起关键作用。然而，如何动态且精确地调节该信号传导途径仍是未知的。在这里，我们报告两个U框类型E3泛素连接酶，PUB25和PUB26，正调控拟南芥的冷冻耐受性。PUB25和PUB26多聚泛素化MYB15是CBF依赖的冷信号通路的转录阻遏物，导致MYB15降解，从而增强了冷胁迫下CBF的表达。此外，冷激活的OST1在保守的苏氨酸残基上特异性磷酸化PUB25和PUB26，增强其E3活性并促进冷诱导的MYB15降解。因此，我们的结果阐明了OST1-PUB25 / 26模块在通过调节负调节剂MYB15的稳态来调节冷反应的持续时间和幅度中的调节作用。
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