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Plasmid Distribution among Escherichia coli from Livestock and Associated Wastewater: Unraveling Factors That Shape the Presence of Genes Conferring Third-Generation Cephalosporin Resistance
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2019-10-01 , DOI: 10.1021/acs.est.9b03486 Lisa Tymensen 1 , Calvin W. Booker 2 , Sherry J. Hannon 2 , Shaun R. Cook 1, 3 , Cassandra C. Jokinen 1 , Rahat Zaheer 3 , Ron Read 4 , Patrick Boerlin 5 , Tim A. McAllister 3
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2019-10-01 , DOI: 10.1021/acs.est.9b03486 Lisa Tymensen 1 , Calvin W. Booker 2 , Sherry J. Hannon 2 , Shaun R. Cook 1, 3 , Cassandra C. Jokinen 1 , Rahat Zaheer 3 , Ron Read 4 , Patrick Boerlin 5 , Tim A. McAllister 3
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A key concern with agricultural wastewater storage ponds is that they may provide an environment conducive for horizontal exchange of antibiotic resistance genes (ARGs), thereby facilitating the emergence of antibiotic resistant pathogens. Central to this exchange are mobile genetic elements like plasmids; yet, the factors shaping their presence in agricultural environments remain poorly understood. Here, using Escherichia coli as a model bacterium, we examined genetic backgrounds and plasmid profiles of generic fecal and wastewater isolates and those possessing blaCTX-M and blaCMY-2 genes (which confer resistance to third-generation cephalosporins) to delineate factors shaping the environmental persistence of plasmid-associated ARGs in beef cattle feedlots. The wastewater environment exerted minimal influence on plasmid repertoires, as the number of plasmids and distribution of different incompatibility groups did not differ between generic fecal and wastewater isolates. The blaCTX-M and blaCMY-2 genes were associated with IncF and IncA/C plasmids, respectively, and host isolates possessing these ARGs had fewer plasmids than generic isolates, suggesting ARG-bearing plasmids may associate predominantly with such hosts to compensate for the metabolic burden imposed by these plasmids. Phylogeny also appeared to be a factor for blaCTX-M genes, as their bacterial hosts were restricted to particular genetic lineages, including the environmentally adapted ET-1 clade, as noted previously for these genes. Ultimately, these findings have important implications for evaluating human health risks of agricultural wastewater with respect to environmental persistence of ARGs and may help identify options for improving wastewater treatment.
中文翻译：
禽畜和相关废水中大肠杆菌的质粒分布：阐明赋予第三代头孢菌素抗性基因的存在的解开因素
农业废水储存池的一个关键问题是它们可以提供有利于抗生素抗性基因（ARGs）水平交换的环境，从而促进抗生素抗性病原体的出现。这种交换的中心是可移动的遗传元件，如质粒。然而，影响它们在农业环境中存在的因素仍然知之甚少。在这里，我们使用大肠杆菌作为模型细菌，研究了普通粪便和废水分离株以及具有bla CTX-M和bla CMY-2的分离株的遗传背景和质粒图谱基因（赋予对第三代头孢菌素的抗性）来描述影响肉牛饲养场中与质粒相关的ARG的环境持久性的因素。废水环境对质粒库的影响最小，因为普通粪便和废水分离株之间质粒的数量和不同不相容基团的分布没有差异。所述BLA CTX-M和BLA CMY-2基因与INCF和INCA / C的质粒分别相关联，和主机分离物具有这些的ARG具有比通用的菌株的质粒更少，这表明ARG-轴承质粒可以与这样的主机，以补偿主要关联这些质粒施加的代谢负担。系统发育也似乎是导致bla CTX-M基因，因为它们的细菌宿主仅限于特定的遗传谱系，包括先前针对这些基因指出的环境适应性ET-1进化枝。最终，这些发现对于评估ARGs在环境方面的持久性对农业废水的人类健康风险具有重要意义，并且可能有助于确定改善废水处理的方案。
更新日期：2019-10-01
中文翻译：
禽畜和相关废水中大肠杆菌的质粒分布：阐明赋予第三代头孢菌素抗性基因的存在的解开因素
农业废水储存池的一个关键问题是它们可以提供有利于抗生素抗性基因（ARGs）水平交换的环境，从而促进抗生素抗性病原体的出现。这种交换的中心是可移动的遗传元件，如质粒。然而，影响它们在农业环境中存在的因素仍然知之甚少。在这里，我们使用大肠杆菌作为模型细菌，研究了普通粪便和废水分离株以及具有bla CTX-M和bla CMY-2的分离株的遗传背景和质粒图谱基因（赋予对第三代头孢菌素的抗性）来描述影响肉牛饲养场中与质粒相关的ARG的环境持久性的因素。废水环境对质粒库的影响最小，因为普通粪便和废水分离株之间质粒的数量和不同不相容基团的分布没有差异。所述BLA CTX-M和BLA CMY-2基因与INCF和INCA / C的质粒分别相关联，和主机分离物具有这些的ARG具有比通用的菌株的质粒更少，这表明ARG-轴承质粒可以与这样的主机，以补偿主要关联这些质粒施加的代谢负担。系统发育也似乎是导致bla CTX-M基因，因为它们的细菌宿主仅限于特定的遗传谱系，包括先前针对这些基因指出的环境适应性ET-1进化枝。最终，这些发现对于评估ARGs在环境方面的持久性对农业废水的人类健康风险具有重要意义，并且可能有助于确定改善废水处理的方案。
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