Self-Assembling Coiled-Coil Peptide Nanotubes with Biomolecular Cargo Encapsulation,ACS Biomaterials Science & Engineering

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Self-Assembling Coiled-Coil Peptide Nanotubes with Biomolecular Cargo Encapsulation
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-09-30 , DOI: 10.1021/acsbiomaterials.9b01304
Monessha Nambiar ₁ , Manish Nepal ₁ , Jean Chmielewski ₁

Affiliation

We report a coiled-coil trimeric peptide based on the known GCN4 zipper motif that self-assembles into nanotubes with diameters on the order of a few hundred nanometers to a micron. The dimensional morphology of these tubular structures was observed to be tunable by altering the properties of the buffer during assembly. Structural evidence from X-ray scattering and electron microscopy suggest that tube assembly takes place in structural tiers leading to a hexagonal close-packed arrangement of the coiled-coils. The hollow tubes were observed to selectively encapsulate fluorescein-labeled anionic dextran, as a result of electrostatic interactions between the inner tube surface and negatively charged cargo. The ability of these nanotubes to house biomolecular cargo endows them with potential for molecular storage and drug delivery applications.

中文翻译：

具有生物分子货物封装的自组装螺旋线圈肽纳米管

我们报告了基于已知的GCN4拉链基序的卷曲螺旋三聚体肽，该肽可自组装成直径为几百纳米到微米的纳米管。通过在组装过程中改变缓冲液的性质，可以观察到这些管状结构的尺寸形态是可调节的。X射线散射和电子显微镜的结构证据表明，管组装发生在结构层中，导致盘绕线圈呈六边形密堆积。由于内管表面和带负电荷的货物之间的静电相互作用，观察到中空管选择性地封装了荧光素标记的阴离子葡聚糖。这些纳米管容纳生物分子货物的能力赋予它们在分子存储和药物递送应用方面的潜力。

更新日期：2019-09-30

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